[HTML][HTML] Cryo-electron tomography: the resolution revolution and a surge of in situ virological discoveries

Y Hong, Y Song, Z Zhang, S Li - Annual Review of Biophysics, 2023 - annualreviews.org
The recent proliferation of cryo-electron tomography (cryo-ET) techniques has led to the cryo-
ET resolution revolution. Meanwhile, significant efforts have been made to improve the …

[HTML][HTML] Biomolecular condensates: insights into early and late steps of the HIV-1 replication cycle

F Di Nunzio, VN Uversky, AJ Mouland - Retrovirology, 2023 - Springer
A rapidly evolving understanding of phase separation in the biological and physical
sciences has led to the redefining of virus-engineered replication compartments in many …

Defining basic rules for hardening influenza A virus liquid condensates

TA Etibor, S Vale-Costa, S Sridharan, D Brás, I Becher… - Elife, 2023 - elifesciences.org
In biological systems, liquid and solid-like biomolecular condensates may contain the same
molecules but their behaviour, including movement, elasticity, and viscosity, is different on …

[HTML][HTML] Melatonin: Regulation of viral phase separation and Epitranscriptomics in post-acute sequelae of COVID-19

D Loh, RJ Reiter - International journal of molecular sciences, 2022 - mdpi.com
The relentless, protracted evolution of the SARS-CoV-2 virus imposes tremendous pressure
on herd immunity and demands versatile adaptations by the human host genome to counter …

Virus structures revealed by advanced cryoelectron microscopy methods

D Zhu, D Cao, X Zhang - Structure, 2023 - cell.com
Before the resolution revolution, cryoelectron microscopy (cryo-EM) single-particle analysis
(SPA) already achieved resolutions beyond 4 Å for certain icosahedral viruses, enabling ab …

[HTML][HTML] The cryoEM structure of the Hendra henipavirus nucleoprotein reveals insights into paramyxoviral nucleocapsid architectures

TC Passchier, JBR White, DP Maskell, MJ Byrne… - Scientific Reports, 2024 - nature.com
We report the first cryoEM structure of the Hendra henipavirus nucleoprotein in complex with
RNA, at 3.5 Å resolution, derived from single particle analysis of a double …

[HTML][HTML] Structural characterization of Thogoto Virus nucleoprotein provides insights into viral RNA encapsidation and RNP assembly

A Dick, V Mikirtumov, J Fuchs, F Krupp, D Olal, E Bendl… - Structure, 2024 - cell.com
Orthomyxoviruses, such as influenza and thogotoviruses, are important human and animal
pathogens. Their segmented viral RNA genomes are wrapped by viral nucleoproteins (NPs) …

Rules for hardening influenza A virus liquid condensates

TA Etibor, S Vale-Costa, S Sridharan, D Brás, I Becher… - BioRxiv, 2022 - biorxiv.org
Multiple viral infections form biomolecular condensates in the host cell to compartmentalize
viral reactions. Accumulating evidence indicates that these viral condensates may be …

Spatial resolution of virus replication: RSV and cytoplasmic inclusion bodies

J Risso-Ballester, MA Rameix-Welti - Advances in Virus Research, 2023 - Elsevier
Abstract Respiratory Syncytial Virus (RSV) is a major cause of respiratory illness in young
children, elderly and immunocompromised individuals worldwide representing a severe …

In Situ Imaging of Virus-Infected Cells by Cryo-Electron Tomography: An Overview

S Vijayakrishnan - Virus Infected Cells, 2023 - Springer
Cryo-electron tomography (cryo-ET) has emerged as a powerful tool in structural biology to
study viruses and is undergoing a resolution revolution. Enveloped viruses comprise several …