[PDF][PDF] FastPCR software for PCR primer and probe design and repeat search

R Kalendar, D Lee, AH Schulman - Genes, genomes and …, 2009 - researchgate.net
Reproducible and target-specific polymerase chain reaction (PCR) amplification relies on
several interrelated factors of which primer design is central. Here, we describe new free …

[HTML][HTML] Java web tools for PCR, in silico PCR, and oligonucleotide assembly and analysis

R Kalendar, D Lee, AH Schulman - Genomics, 2011 - Elsevier
The polymerase chain reaction is fundamental to molecular biology and is the most
important practical molecular technique for the research laboratory. We have developed and …

FastPCR software for PCR, in silico PCR, and oligonucleotide assembly and analysis

R Kalendar, D Lee, AH Schulman - DNA cloning and assembly methods, 2014 - Springer
This chapter introduces the software FastPCR as an integrated tools environment for PCR
primer and probe design. It also predicts oligonucleotide properties based on experimental …

In silicocomparative analysis of SSR markers in plants

FC Victoria, LC da Maia, AC de Oliveira - BMC plant biology, 2011 - Springer
Background The adverse environmental conditions impose extreme limitation to growth and
plant development, restricting the genetic potential and reflecting on plant yield losses. The …

The BiSearch web server

T Arányi, A Váradi, I Simon, GE Tusnády - BMC bioinformatics, 2006 - Springer
Background A large number of PCR primer-design softwares are available online. However,
only very few of them can be used for the design of primers to amplify bisulfite-treated DNA …

Improved assay-dependent searching of nucleic acid sequence databases

JD Gans, M Wolinsky - Nucleic acids research, 2008 - academic.oup.com
Nucleic acid-based biochemical assays are crucial to modern biology. Key applications,
such as detection of bacterial, viral and fungal pathogens, require detailed knowledge of …

GENOMEMASKER package for designing unique genomic PCR primers

R Andreson, E Reppo, L Kaplinski, M Remm - BMC bioinformatics, 2006 - Springer
Background The design of oligonucleotides and PCR primers for studying large genomes is
complicated by the redundancy of sequences. The eukaryotic genomes are particularly …

Quantification of Human Oral and Fecal Streptococcus parasanguinis by Use of Quantitative Real-Time PCR Targeting the groEL Gene

Q Chen, G Wu, H Chen, H Li, S Li, C Zhang… - Frontiers in …, 2019 - frontiersin.org
Two pairs of species-specific PCR primers targeting the housekeeping groEL gene, Spa146f-
Spa525r and Spa93f-Spa525r, were designed to quantify human oral and fecal …

[图书][B] Methods and software for predicting PCR failure rate in large genomes

R Andreson - 2008 - bioinfo.ut.ee
Modern genomic technologies allow studying thousands of genomic regions from each DNA
sample. Many of these technologies rely on methodology called polymerase chain reaction …

[HTML][HTML] Mining and analysis of simple sequence repeats in the chloroplast genomes of genus Vigna

N Shukla, H Kuntal, A Shanker, SN Sharma - Biotechnology Research and …, 2018 - Elsevier
The genus Vigna is distributed throughout the world and it is phylogenetically closely related
to agriculturally important crops. Simple sequence repeats (SSRs) are defined as sequence …