Mass spectrometry is a central technology in the life sciences, providing our most
comprehensive account of the molecular inventory of the cell. In parallel with developments …

The prevailing current view of protein folding is the thermodynamic hypothesis, under which
the native folded conformation of a protein corresponds to the global minimum of Gibbs free …

Protein engineering often targets binding pockets or active sites which are enriched in
epistasis—nonadditive interactions between amino acid substitutions—and where the …

Stability of proteins at high temperature has been a topic of interest for many years, as this
attribute is favourable for applications ranging from therapeutics to industrial chemical …

Differential scanning fluorimetry (DSF) is a widely used technique for determining the
apparent melting temperature (Tma) of a purified protein. Here, we present a protocol for …

ABSTRACT P4-ATPases flip lipids from the exoplasmic to cytoplasmic leaflet of cell
membranes, a property crucial for many biological processes. Mutations in P4-ATPases are …

Accurate predictions from models based on physical principles are the ultimate metric of our
biophysical understanding. Although there has been stunning progress toward structure …

Proteins are large biomolecules with a specific structure that is composed of one or more
long amino acid chains. Correct protein structures are directly linked to their correct function …

Flexible in vitro methods alter the course of biological discoveries. Differential Scanning
Fluorimetry (DSF) is a particularly versatile technique which reports protein thermal …

Abstract Src-homology-2-domain-containing protein tyrosine phosphatase-2 (SHP2) is a
signaling enzyme whose activity is governed by an equilibrium between autoinhibited and …