Background Technologies for making and manipulating DNA have enabled advances in
biology ever since the discovery of the DNA double helix. But introducing site-specific …

Gene therapy has historically been defined as the addition of new genes to human cells.
However, the recent advent of genome-editing technologies has enabled a new paradigm in …

Over the past decade, the Rosetta biomolecular modeling suite has informed diverse
biological questions and engineering challenges ranging from interpretation of low …

Engineered nucleases can be used to induce site‐specific double‐strand breaks (DSB s) in
plant genomes. Thus, homologous recombination (HR) can be enhanced and targeted …

Recent developments in computational chemistry and biology have come together in the
“inside‐out” approach to enzyme engineering. Proteins have been designed to catalyze …

Genome-editing, a recent technological advancement in the field of life sciences, is one of
the great examples of techniques used to explore the understanding of the biological …

The importance of safer approaches for gene therapy has been underscored by a series of
severe adverse events (SAEs) observed in patients involved in clinical trials for Severe …

Site-specific selfish genes exploit host functions to copy themselves into a defined target
DNA sequence, and include homing endonuclease genes, group II introns and some LINE …

The efficient repair of double-strand breaks (DSBs) in genomic DNA is important for the
survival of all organisms. In recent years, basic mechanisms of DSB repair in somatic plant …

The ability to achieve site-specific manipulation of the mammalian genome has widespread
implications for basic and applied research. Gene targeting is a process in which a DNA …