[HTML][HTML] Nanopore sequencing technology, bioinformatics and applications
Rapid advances in nanopore technologies for sequencing single long DNA and RNA
molecules have led to substantial improvements in accuracy, read length and throughput …
molecules have led to substantial improvements in accuracy, read length and throughput …
RNA sequencing: the teenage years
Over the past decade, RNA sequencing (RNA-seq) has become an indispensable tool for
transcriptome-wide analysis of differential gene expression and differential splicing of …
transcriptome-wide analysis of differential gene expression and differential splicing of …
Highly accurate long-read HiFi sequencing data for five complex genomes
T Hon, K Mars, G Young, YC Tsai, JW Karalius… - Scientific data, 2020 - nature.com
Abstract The PacBio® HiFi sequencing method yields highly accurate long-read sequencing
datasets with read lengths averaging 10–25 kb and accuracies greater than 99.5%. These …
datasets with read lengths averaging 10–25 kb and accuracies greater than 99.5%. These …
SQANTI: extensive characterization of long-read transcript sequences for quality control in full-length transcriptome identification and quantification
M Tardaguila, L De La Fuente, C Marti… - Genome …, 2018 - genome.cshlp.org
High-throughput sequencing of full-length transcripts using long reads has paved the way
for the discovery of thousands of novel transcripts, even in well-annotated mammalian …
for the discovery of thousands of novel transcripts, even in well-annotated mammalian …
[PDF][PDF] Gene body methylation in plants: mechanisms, functions, and important implications for understanding evolutionary processes
Gene body methylation (gbM) is an epigenetic mark where gene exons are methylated in
the CG context only, as opposed to CHG and CHH contexts (where H stands for A, C, or T) …
the CG context only, as opposed to CHG and CHH contexts (where H stands for A, C, or T) …
Analysis of transcriptome and epitranscriptome in plants using PacBio Iso-Seq and nanopore-based direct RNA sequencing
L Zhao, H Zhang, MV Kohnen, KVSK Prasad… - Frontiers in …, 2019 - frontiersin.org
Nanopore sequencing from Oxford Nanopore Technologies (ONT) and Pacific BioSciences
(PacBio) single-molecule real-time (SMRT) long-read isoform sequencing (Iso-Seq) are …
(PacBio) single-molecule real-time (SMRT) long-read isoform sequencing (Iso-Seq) are …
Best practices on the differential expression analysis of multi-species RNA-seq
Advances in transcriptome sequencing allow for simultaneous interrogation of differentially
expressed genes from multiple species originating from a single RNA sample, termed dual …
expressed genes from multiple species originating from a single RNA sample, termed dual …
Illuminating the dark side of the human transcriptome with long read transcript sequencing
Background The human transcriptome annotation is regarded as one of the most complete
of any eukaryotic species. However, limitations in sequencing technologies have biased the …
of any eukaryotic species. However, limitations in sequencing technologies have biased the …
Methodologies for transcript profiling using long-read technologies
S Oikonomopoulos, A Bayega, S Fahiminiya… - Frontiers in …, 2020 - frontiersin.org
RNA sequencing using next-generation sequencing technologies (NGS) is currently the
standard approach for gene expression profiling, particularly for large-scale high-throughput …
standard approach for gene expression profiling, particularly for large-scale high-throughput …
RNA sequencing and its applications in cancer and rare diseases
S Ergin, N Kherad, M Alagoz - Molecular Biology Reports, 2022 - Springer
With the invention of RNA sequencing over a decade ago, diagnosis and identification of the
gene-related diseases entered a new phase that enabled more accurate analysis of the …
gene-related diseases entered a new phase that enabled more accurate analysis of the …