Chromosome structure in mammals is thought to regulate transcription by modulating three-
dimensional interactions between enhancers and promoters, notably through CTCF …

Human glioblastomas harbour a subpopulation of glioblastoma stem cells that drive
tumorigenesis. However, the origin of intratumoural functional heterogeneity between …

The broad availability of amplified DNA molecules enabled the development of many
powerful applications in molecular biology techniques. Currently, a variety of DNA …

Large-scale genetic analysis of lethal phenotypes has elucidated the molecular
underpinnings of many biological processes. Using the bacterial clustered regularly …

Intratumoral heterogeneity arises through the evolution of genetically diverse subclones
during tumor progression. However, it remains unknown whether cells within single genetic …

Insertional mutagenesis has been used as a functional forward genetics screen for the
identification of novel genes involved in the pathogenesis of human cancers. Different …

Targeting membrane proteins could improve the efficacy of T cell–based immunotherapies.
To facilitate the identification of T cell targets, we developed a hybrid genetic screening …

Despite developments in targeted gene sequencing and whole-genome analysis
techniques, the robust detection of all genetic variation, including structural variants, in and …

Pancreatic ductal adenocarcinoma (PDA) remains a lethal malignancy despite much
progress concerning its molecular characterization. PDA tumours harbour four signature …

Transposon insertion sequencing is a high-throughput technique for assaying large libraries
of otherwise isogenic transposon mutants providing insight into gene essentiality, gene …