Single-molecule localization microscopy (SMLM) describes a family of powerful imaging
techniques that dramatically improve spatial resolution over standard, diffraction-limited …

Access to DNA is a prerequisite to the execution of essential cellular processes that include
transcription, replication, chromosomal segregation, and DNA repair. How the proteins that …

In eukaryotes, higher-order chromatin organization is spatiotemporally regulated as
domains, for various cellular functions. However, their physical nature in living cells remains …

Liquid–liquid phase separation (LLPS) is an important mechanism that helps explain the
membraneless compartmentalization of the nucleus. Because chromatin compaction and …

Fluorescent proteins (FP) are frequently used for studying proteins inside cells. In advanced
fluorescence microscopy, FPs can report on additional intracellular variables. One variable …

Single-molecule tracking (SMT) has emerged as the dominant technology to investigate the
dynamics of chromatin-transcription factor (TF) interactions. How long a TF needs to bind to …

Dynamic chromatin behavior plays a critical role in various genome functions. However, it
remains unclear how chromatin behavior changes during interphase, where the nucleus …

How chromatin dynamics relate to transcriptional activity remains poorly understood. Using
single-molecule tracking, coupled with machine learning, we show that histone H2B and …

Nuclear DNA in eukaryotes is wrapped around histone proteins to form nucleosomes on a
chromatin fiber. Dynamic folding of the chromatin fiber into loops and variations in the …

Genomic information is encoded on long strands of DNA, which are folded into chromatin
and stored in a tiny nucleus. Nuclear chromatin is a negatively charged polymer composed …