Natto, a fermented soybean product, has been consumed as a traditional food in Japan for
thousands of years. Nattokinase (NK), a potent blood-clot dissolving protein used for the …

Nattokinase (NK, also known as subtilisin NAT)(EC 3.4. 21.62) is one of the most
considerable extracellular enzymes produced by Bacillus subtilis natto. The main interest …

Background Many methods are available for the concentration of proteins; however, most
are not easily scalable due to costs, the need of specialized instruments and skilled workers …

The increasing interest of industrial enzymes demands for development of new downstream
strategies for maximizing enzyme recovery. The significant efforts have been focused on the …

Fibrinolytic enzymes are proteases responsible for cleavage of fibrin mesh in thrombus clots,
which are the primary causative agents in cardiovascular diseases. Developing safe …

With the dramatic increase in mortality of cardiovascular diseases (CVDs) caused by
thrombus, this has sparked an interest in seeking more effective thrombolytic drugs or …

In recent years, a big trend has been the development of rapid, green, efficient, economical,
and scalable approaches for the separation and purification of bioactive molecules from …

Protein biopharmaceuticals, among which interferon alpha-2b (IFNα-2b) that can be used in
the treatment of chronic hepatitis C and hairy cell leukemia, have become an indispensable …

Three-phase partitioning (TPP) is rapidly developing as a novel bioseparation technique for
the separation and purification of biomolecules. TPP has been applied in a wide range of …

Curcuminoids, the active components of dried rhizome of Curcuma longa have been
extracted using batch extraction and three-phase partitioning (TPP) process. The effect of …