Over the past 20 years, protein engineering has been extensively used to improve and
modify the fundamental properties of fluorescent proteins (FPs) with the goal of adapting …

Highlights•Microfluidics complements high sensitivity molecular tools, allowing single cell
analysis.•Cells can be directly manipulated by a variety of fields, with forces equivalent …

The low photostability of fluorescent proteins is a limiting factor in many applications of
fluorescence microscopy. Here we present StayGold, a green fluorescent protein (GFP) …

Although StayGold is a bright and highly photostable fluorescent protein, its propensity for
obligate dimer formation may hinder applications in molecular fusion and membrane …

The approximately linear scaling of fluorescence quantum yield (ϕ) with fluorescence
lifetime (τ) in fluorescent proteins (FPs) has inspired engineering of brighter fluorophores …

Fluorescent proteins (FPs) have become ubiquitous tools for biological research and
concomitantly they are intriguing molecules that are amenable to study with a wide range of …

Density Peaks (DP) is a recently proposed clustering algorithm that has distinctive
advantages over existing clustering algorithms. It has already been used in a wide range of …

We report a high-throughput screening method that allows diverse genotypes and
corresponding phenotypes to be imaged in individual cells. We achieve genotyping by …

Abstract Density Peak Clustering (DPC) is a popular state-of-the-art clustering algorithm,
which requires pairwise (dis) similarity of data objects to detect arbitrary shaped clusters …

Fluorescent biosensors are important measurement tools for in vivo quantification of pH,
concentrations of metal ions and other analytes, and physical parameters such as …