PIFE was first used as an acronym for protein-induced fluorescence enhancement, which
refers to the increase in fluorescence observed upon the interaction of a fluorophore, such …

Membrane proteins play key roles in most crucial cellular processes, ranging from cell-to-
cell communication to signaling processes. Despite recent improvements, the expression of …

Fluorescence-based sensors play a fundamental role in biological research. These sensors
can be based on fluorescent proteins, fluorescent probes or they can be hybrid systems. The …

The adenosine triphosphate (ATP)-binding cassette (ABC) importer GlnPQ from
Lactococcus lactis has two sequential covalently linked substrate-binding domains (SBDs) …

The substrate‐binding domain 2 (SBD2) is an important part of the bacterial glutamine
(GLN) transporter and mediates binding and delivery of GLN to the transporter translocation …

Ligand binding and conformational changes of biomacromolecules play a central role in the
regulation of cellular processes. It is important to understand how both are coupled and what …

With an estimate of hundred thousands of protein molecules per cell and the number of
metabolites several orders of magnitude higher, protein-metabolite interactions are …

Pneumococcal pneumonia causes cytotoxicity in the lung parenchyma but the underlying
mechanism involves multiple factors contributing to cell death. Here, we discovered that …

Although fluorescent dyes are among the most widely used bioimaging techniques as they
provide detailed information on a single biomolecular level with high spatial and temporal …

Although fluorescent dyes are among the most widely used bioimaging techniques as they
provide detailed information on a single biomolecular level with high spatial and temporal …