[HTML][HTML] The consequences of recurrent genetic and epigenetic variants in human pluripotent stem cells

PW Andrews, I Barbaric, N Benvenisty, JS Draper… - Cell Stem Cell, 2022 - cell.com
It is well established that human pluripotent stem cells (hPSCs) can acquire genetic and
epigenetic changes during culture in vitro. Given the increasing use of hPSCs in research …

Biomanufacturing human tissues via organ building blocks

KJ Wolf, JD Weiss, SGM Uzel, MA Skylar-Scott… - Cell Stem Cell, 2022 - cell.com
The construction of human organs on demand remains a tantalizing vision to solve the
organ donor shortage. Yet, engineering tissues that recapitulate the cellular and …

A reference human induced pluripotent stem cell line for large-scale collaborative studies

CB Pantazis, A Yang, E Lara, JA McDonough… - Cell stem cell, 2022 - cell.com
Human induced pluripotent stem cell (iPSC) lines are a powerful tool for studying
development and disease, but the considerable phenotypic variation between lines makes it …

How can clinical safety and efficacy concerns in stem cell therapy for spinal cord injury be overcome?

N Hejrati, R Wong, M Khazaei… - Expert Opinion on …, 2023 - Taylor & Francis
Introduction Spinal cord injury (SCI) can lead to severe neurological dysfunction. Despite
scientific and medical advances, clinically effective regenerative therapies including stem …

Complex regulatory networks influence pluripotent cell state transitions in human iPSCs

TD Arthur, JP Nguyen, A D'Antonio-Chronowska… - Nature …, 2024 - nature.com
Stem cells exist in vitro in a spectrum of interconvertible pluripotent states. Analyzing
hundreds of hiPSCs derived from different individuals, we show the proportions of these …

DPPA5A suppresses the mutagenic TLS and MMEJ pathways by modulating the cryptic splicing of Rev1 and Polq in mouse embryonic stem cells

F Jiang, L Wang, Y Dong, W Nie… - Proceedings of the …, 2023 - National Acad Sciences
Genetic alterations are often acquired during prolonged propagation of pluripotent stem
cells (PSCs). This ruins the stem cell quality and hampers their full applications …

[HTML][HTML] Feeder-free culture of human pluripotent stem cells drives MDM4-mediated gain of chromosome 1q

D Stavish, CJ Price, G Gelezauskaite, H Alsehli… - Stem Cell Reports, 2024 - cell.com
Culture-acquired variants in human pluripotent stem cells (hPSCs) hinder their applications
in research and clinic. However, the mechanisms that underpin selection of variants remain …

Carnegie in 4D? Stem-cell-based models of human embryo development

B Sozen, D Conkar, JV Veenvliet - Seminars in cell & developmental …, 2022 - Elsevier
How cells build embryos is still a major mystery. Many unresolved questions require the
study of the processes that pattern and shape the embryo in live specimens, in toto, across …

Somatic mutations alter the differentiation outcomes of iPSC-derived neurons

P Puigdevall, J Jerber, P Danecek, S Castellano… - Cell genomics, 2023 - cell.com
The use of induced pluripotent stem cells (iPSC) as models for development and human
disease has enabled the study of otherwise inaccessible tissues. A remaining challenge in …

Pluripotent stem cell strategies for rebuilding the human brain

F Limone, JR Klim, DA Mordes - Frontiers in Aging Neuroscience, 2022 - frontiersin.org
Neurodegenerative disorders have been extremely challenging to treat with traditional drug-
based approaches and curative therapies are lacking. Given continued progress in stem cell …