Cancer metabolism is significantly altered from normal cellular metabolism allowing cancer
cells to adapt to changing microenvironments and maintain high rates of proliferation. In the …

To understand consumer dietary requirements and resource use across ecosystems,
researchers have employed a variety of methods, including bulk stable isotope and fatty acid …

The chemical reactions that formed the building blocks of life at origins required catalysts,
whereby the nature of those catalysts influenced the type of products that accumulated …

A plant pathway that initiates with the formation of citramalate from pyruvate and acetyl-CoA
by citramalate synthase (CMS) is shown to contribute to the synthesis of α-ketoacids and …

Photosynthesis is the biological process that converts solar energy to biomass, bio-products,
and biofuel. It is the only major natural solar energy storage mechanism on Earth. To satisfy …

Obligatory anaerobic bacteria are major contributors to the overall metabolism of soil and
the human gut. The metabolic pathways of these bacteria remain, however, poorly …

Bio-based production of chemicals from renewable resources is becoming increasingly
important for sustainable chemical industry. In this study, Escherichia coli was metabolically …

Radioactive and stable isotopes have been applied for decades to elucidate metabolic
pathways and quantify carbon flow in cellular systems using mass and isotope balancing …

Highlights•Parallel labeling experiments with multiple tracers improve resolution of pathway
fluxes.•13 C-metabolic flux analysis is increasingly applied to non-model organisms.• …

Microbes have complex metabolic pathways that can be investigated using biochemistry
and functional genomics methods. One important technique to examine cell central …