Validation of suitable reference genes by various algorithms for gene expression analysis in Isodon rubescens under different abiotic stresses

C Lian, B Zhang, J Yang, JX Lan, H Yang, K Guo… - Scientific Reports, 2022 - nature.com
Abstract Isodon rubescens (Hemsley) H. Hara (Lamiaceae) is a traditional Chinese
medicine plant that has been used to treat various human diseases. Oridonin is one of the …

Biochemical, anatomical, and pharmacological characterization of calcitonin-type neuropeptides in starfish: discovery of an ancient role as muscle relaxants

W Cai, CH Kim, HJ Go, M Egertová… - Frontiers in …, 2018 - frontiersin.org
Calcitonin (CT) is a peptide hormone released by the thyroid gland that regulates blood
Ca2+ levels in mammals. The CT gene is alternatively spliced, with one transcript encoding …

Comparison of Reliable Reference Genes Following Different Hormone Treatments by Various Algorithms for qRT-PCR Analysis of Metasequoia

JJ Wang, S Han, W Yin, X Xia, C Liu - International Journal of Molecular …, 2018 - mdpi.com
Quantitative reverse transcription polymerase chain reaction (qRT-PCR) is the most
sensitive technique for evaluating gene expression levels. Choosing appropriate reference …

Verify the Function of a Potential Growth-Regulating Gene in Marine Bivalve Using a Candidate Model Organism Mulinia lateralis

C Wang, L Kong, S Lian, Z Yang, D Meng, M Li… - Journal of Ocean …, 2023 - Springer
A better understanding of genetic bases of growth regulation is essential for bivalve
breeding, which is helpful to improve the yield of the commercially important bivalves. While …

Practical Euthanasia Method for Common Sea Stars (Asterias rubens) That Allows for High-Quality RNA Sampling

SJ Wahltinez, KJ Kroll, EA Nunamaker, ND Denslow… - Animals, 2021 - mdpi.com
Simple Summary Sea stars are iconic marine invertebrates and are important for
maintaining the biodiversity in their ecosystems. As humans, we interact with sea stars when …

Role of smoothened and sfrp genes in Eupentacta fraudatrix regeneration

A Girich, K Sadriev, L Frolova… - Wound Repair and …, 2023 - Wiley Online Library
The secreted frizzled‐related proteins (sfrp) and smoothened (smo) genes and their
possible role in the regeneration of internal organs in the holothurian Eupentacta fraudatrix …

Validation of reference genes for gene expression analysis in melanin‐injected black tiger shrimp (Penaeus monodon)

HNT Phan, HLT Nguyen, HG Vu… - Aquaculture …, 2022 - Wiley Online Library
The expression stability of four potential reference genes (EF1‐α, β‐actin, GAPDH and
SubF0) was evaluated to normalize the expression of six immune‐related genes (proPO1 …

Reference genes for expression studies in different developmental stages of Oryctes rhinoceros, the coconut rhinoceros beetle

K Arvind, G Antony, MK Rajesh… - Journal of Asia-Pacific …, 2023 - Elsevier
Abstract Oryctes rhinoceros L.(Coleoptera: Scarabaeidae), the coconut rhinoceros beetle
(CRB), is a primary pest of coconut in South and Southeast Asia and the Pacific Islands. The …

Selection and validation of reference genes for RT-qPCR analysis of different organs at various development stages in Caragana intermedia

J Liu, C Yang, M Bai, F Yan, H Qin, R Wang… - Open Life …, 2022 - degruyter.com
Reverse transcription quantitative PCR (RT-qPCR) is a technique widely used to investigate
the expression of genes. An appropriate reference gene (RG) is essential for RT-qPCR …

The choanoflagellate translational machinery

P Armitage - 2019 - eprints.hud.ac.uk
Introduction Choanoflagellates are the closest living, single celled eukaryotic relative to the
Metazoa. As such, they provide an ideal model organism to investigate evolution of …