Microbeam methodologies as powerful tools in manganese hyperaccumulation research: present status and future directions

DR Fernando, A Marshall, AJM Baker… - Frontiers in Plant …, 2013 - frontiersin.org
Microbeam studies over the past decade have garnered unique insight into manganese
(Mn) homeostasis in plant species that hyperaccumulate this essential mineral micronutrient …

Salt sensitivity in chickpea (Cicer arietinum L.): ions in reproductive tissues and yield components in contrasting genotypes

L Kotula, HA Khan, J Quealy, NC Turner… - Plant, cell & …, 2015 - Wiley Online Library
The reproductive phase in chickpea (C icer arietinum L.) is affected by salinity, but little is
known about the underlying cause. We investigated whether high concentrations of Na+ and …

The X-ray microanalysis of frozen-hydrated sections in scanning electron microscopy: an evaluation

BL Gupta, TA Hall - Tissue and Cell, 1981 - Elsevier
The present status of the technique to measure concentrations of electrolyte elements and
dry mass in∼ 1 μm thick frozen-hydrated sections of soft biological tissues with electron …

Manganese accumulation in the leaf mesophyll of four tree species: a PIXE/EDAX localization study

DR Fernando, EJ Bakkaus, N Perrier… - New …, 2006 - Wiley Online Library
Little is known about the spatial distribution of excess manganese (Mn) in the leaves of
tolerant plants. Recently, the first such study of a Mn hyperaccumulator showed that the …

Quantitative X‐ray microanalysis of biological specimens

GM Roomans - Journal of electron microscopy technique, 1988 - Wiley Online Library
Qualitative X‐ray microanalysis of biological specimens requires an approach that is
somewhat different from that used in the materials sciences. The first step is deconvolution …

In-vacuum micro-PIXE analysis of biological specimens in frozen-hydrated state

G Tylko, J Mesjasz-Przybyłowicz… - Nuclear Instruments and …, 2007 - Elsevier
The first in-vacuum measurements of biological specimens in frozen-hydrated state using
proton microbeam have been performed at Materials Research Group, iThemba LABS …

Low temperature scanning electron microscopy: advantages and applications

JA Sargent - Scanning Microscopy, 1988 - digitalcommons.usu.edu
Cryo-preparation of specimens for scanning electron microscopy can be completed within a
few minutes. Chemical fixation and contact with solvents is avoided, levels of specimen …

X‐ray microanalysis of biological material in the frozen‐hydrated state by PIXE

G Tylko, J Mesjasz‐Przybyłowicz… - Microscopy Research …, 2007 - Wiley Online Library
Elemental microanalysis of biological material in the frozen‐hydrated state using in‐vacuum
proton induced X‐ray emission is described for the first time. For this purpose, a …

Micro-PIXE analyses of frozen-hydrated semi-thick biological sections

YD Wang, J Mesjasz-Przybylowicz, G Tylko… - Nuclear Instruments and …, 2013 - Elsevier
Cryo-micro-PIXE system and methodology of microanalysis of frozen-hydrated semi-thick
biological sections is described. A commercially available cryotransfer system used in …

The preparation and X‐ray microanalysis of bulk frozen hydrated vacuolate plant tissue

P Echlin, SE Taylor - Journal of Microscopy, 1986 - Wiley Online Library
SUMMARY A series of modifications have been devised which allow the peak to
background ratio X‐ray analytical method to be used more effectively to measure elemental …