DNA metabarcoding in diet studies: Unveiling ecological aspects in aquatic and terrestrial ecosystems

LL de Sousa, SM Silva, R Xavier - Environmental DNA, 2019 - Wiley Online Library
Effective conservation of species and ecosystems requires the understanding of important
ecological traits, such as dietary habits, food webs, and trophic niches. In diet studies, the …

Who is eating what: diet assessment using next generation sequencing

F Pompanon, BE Deagle, WOC Symondson… - Molecular …, 2012 - Wiley Online Library
The analysis of food webs and their dynamics facilitates understanding of the mechanistic
processes behind community ecology and ecosystem functions. Having accurate techniques …

Replication levels, false presences and the estimation of the presence/absence from eDNA metabarcoding data

GF Ficetola, J Pansu, A Bonin… - Molecular ecology …, 2015 - Wiley Online Library
Environmental DNA (eDNA) metabarcoding is increasingly used to study the present and
past biodiversity. eDNA analyses often rely on amplification of very small quantities or …

Methodological trends and perspectives of animal dietary studies by noninvasive fecal DNA metabarcoding

H Ando, H Mukai, T Komura, T Dewi… - Environmental …, 2020 - Wiley Online Library
Fine‐scale estimation of trophic interactions is an important subject in the field of ecology.
Diet analysis based on fecal DNA metabarcoding has been accepted as a noninvasive …

DNA metabarcoding multiplexing and validation of data accuracy for diet assessment: application to omnivorous diet

M De Barba, C Miquel, F Boyer… - Molecular ecology …, 2014 - Wiley Online Library
Ecological understanding of the role of consumer–resource interactions in natural food webs
is limited by the difficulty of accurately and efficiently determining the complex variety of food …

Promises and pitfalls of using high‐throughput sequencing for diet analysis

A Alberdi, O Aizpurua, K Bohmann… - Molecular ecology …, 2019 - Wiley Online Library
The application of high‐throughput sequencing‐based approaches to DNA extracted from
environmental samples such as gut contents and faeces has become a popular tool for …

Taxon‐specific PCR for DNA barcoding arthropod prey in bat faeces

MRK Zeale, RK Butlin, GLA Barker… - Molecular ecology …, 2011 - Wiley Online Library
The application of DNA barcoding to dietary studies allows prey taxa to be identified in the
absence of morphological evidence and permits a greater resolution of prey identity than is …

Quantitative DNA metabarcoding: improved estimates of species proportional biomass using correction factors derived from control material

AC Thomas, BE Deagle, JP Eveson… - Molecular ecology …, 2016 - Wiley Online Library
DNA metabarcoding is a powerful new tool allowing characterization of species
assemblages using high‐throughput amplicon sequencing. The utility of DNA …

INVITED REVIEW: Molecular analysis of predation: a review of best practice for DNA‐based approaches

RA King, DS Read, M Traugott… - Molecular …, 2008 - Wiley Online Library
Molecular analysis of predation, through polymerase chain reaction amplification of prey
remains within the faeces or digestive systems of predators, is a rapidly growing field …

Carnivore diet analysis based on next‐generation sequencing: application to the leopard cat (Prionailurus bengalensis) in Pakistan

W Shehzad, T Riaz, MA Nawaz, C Miquel… - Molecular …, 2012 - Wiley Online Library
Diet analysis is a prerequisite to fully understand the biology of a species and the
functioning of ecosystems. For carnivores, traditional diet analyses mostly rely upon the …