Super-resolution fluorescence microscopy is a powerful tool to visualize biomolecules and
cellular structures at the nanometer scale. Employing these techniques in living cells has …

Single-molecule super-resolution fluorescence microscopy and single-particle tracking are
two imaging modalities that illuminate the properties of cells and materials on spatial scales …

Pushing the frontier of fluorescence microscopy requires the design of enhanced
fluorophores with finely tuned properties. We recently discovered that incorporation of four …

Fluorescence microscopy relies on dyes that absorb and then emit photons. In addition to
fluorescence, fluorophores can undergo photochemical processes that decrease quantum …

Fluorophores and probes are invaluable for the visualization of the location and dynamics of
gene expression, protein expression, and molecular interactions in complex living systems …

Super-resolution fluorescence imaging by photoactivation or photoswitching of single
fluorophores and position determination (single-molecule localization microscopy, SMLM) …

The past decade has witnessed an explosion in the use of super-resolution fluorescence
microscopy methods in biology and other fields. Single-molecule localization microscopy …

Small-molecule fluorophores are important tools for advanced imaging experiments. We
previously reported a general method to improve small, cell-permeable fluorophores which …

Mammalian genomes are folded into spatial domains, which regulate gene expression by
modulating enhancer-promoter contacts. Here, we review recent studies on the structure …

The controlled switching of fluorophores between non-fluorescent and fluorescent states is
central to every super-resolution fluorescence microscopy (nanoscopy) technique, and the …