With both catalytic and genetic functions, ribonucleic acid (RNA) is perhaps the most
pluripotent chemical species in molecular biology, and its functions are intimately linked to …

Nucleases cleave the phosphodiester bonds of nucleic acids and may be endo or exo,
DNase or RNase, topoisomerases, recombinases, ribozymes, or RNA splicing enzymes. In …

CRISPR/Cas9 uses a guide RNA (gRNA) molecule to execute sequence‐specific DNA
cleavage and it has been widely used for genome editing in many organisms. Modifications …

Ions surround nucleic acids in what is referred to as an ion atmosphere. As a result, the
folding and dynamics of RNA and DNA and their complexes with proteins and with each …

Although RNA is generally thought to be a passive genetic blueprint, some RNA molecules,
called ribozymes, have intrinsic enzyme-like activity—they can catalyse chemical reactions …

The technique of small-probe contact dot surfaces is described as a method for calculating
and displaying the detailed atomic contacts inside or between molecules. It allows one both …

Synthetic oligonucleotide analogs have greatly aided our understanding of several
biochemical processes. Efficient solid-phase and enzyme-assisted synthetic methods and …

▪ Abstract The past few years have seen exciting advances in understanding the structure
and function of catalytic RNA. Crystal structures of several ribozymes have provided detailed …

Biochemical strategies that use a combination of synthetic oligonucleotides, thermostable
DNA polymerases, and DNA ligases can produce large DNA constructs up to 1 megabase in …

Minimal hammerhead ribozymes have been characterized extensively by static and time-
resolved crystallography as well as numerous biochemical analyses, leading to mutually …