Animal cells internalize specific extracellular macromolecules (ligands) by using specialized
cell surface receptors that operate through a complex and highly regulated process known …

Several different gap junction channel subunit isotypes, known as connexins, were
synthesized in a cell-free translation system supplemented with microsomal membranes to …

Tissue-type plasminogen activator (t-PA) is synthesized in mammalian cells as a mixture of
two forms that differ in their extent of N-linked glycosylation. We have investigated the …

Publisher Summary This chapter reviews the role of the enzyme in disulfide bond formation,
and discusses the proposed multi-functionality of protein disulfide-isomerase (PDI). The …

For gap junction channels to function, their subunit proteins, referred to as connexins, have
to be synthesized and inserted into the cell membrane in their native configuration. Like …

We previously demonstrated that a heterotypic complex of the two rat asialoglycoprotein
receptor subunits was assembled during cell-free translation (Sawyer, JT, and D. Doyle …

In the absence of ligand, sodium vanadate causes a time-and dose-dependent loss of up
to~ 50% of the surface galactosyl receptor (GalR) activity in rat hepatocytes at 37° C. The …

Almost all cellular glycoproteins are confined to membrane-limited compartments, with their
oligosaccharide side chains facing away from the cytosol. Extracellular glycoproteins that …

With a few exceptions, all secretory and plasma membrane proteins studied to date are
synthesized in the endoplasmic reticulum (ER) membrane. Then, they are transported by …

As a consequence of safety issues encountered by the use of viral vectors in gene therapy,
there has been a steady increase in the development and application of non-viral vectors …