Membrane proteins are highly diverse in both structure and function and can, therefore,
present different challenges for structure determination. They are biologically important for …

Cryo-electron tomography (cryo-ET) can be used to reconstruct three-dimensional (3D)
volumes, or tomograms, from a series of tilted two-dimensional images of biological objects …

Single-particle electron cryo-microscopy (cryo-EM) is a powerful method for solving the three-
dimensional structures of biological macromolecules. The technological development of …

In 2020, cryo-EM single-particle analysis achieved true atomic resolution thanks to
technological developments in hardware and software. The number of high-resolution …

Our understanding of protein synthesis has been conceptualised around the structure and
function of the bacterial ribosome. This complex macromolecular machine is the target of …

Recent technological advances in cryo electron microscopy (cryo-EM) have led to new
opportunities in the structural biology field. Here we benchmark the performance of two 300 …

Cryo-electron microscopy (cryo-EM) has become a powerful tool to resolve high-resolution
structures of biomacromolecules in solution. However, air-water interface induced preferred …

Cryo–electron microscopy (cryo-EM) continues its remarkable growth as a method for
visualizing biological objects, which has been driven by advances across the entire pipeline …

Detector technology plays a pivotal role in high-resolution and high-throughput cryo-EM
structure determination. Compared with the first-generation, single-electron counting direct …

Over the past decade, technical and methodological improvements in cryogenic electron
microscopy (cryo-EM) single-particle analysis have enabled routine high-resolution …