Efficient xylose utilization is one of the most important pre-requisites for developing an
economic microbial conversion process of terrestrial lignocellulosic biomass into biofuels …

Living organisms evolve complex genetic networks to interact with the environment. Due to
the rapid development of synthetic biology, various modularized genetic parts and units …

The methylotrophic yeast Pichia pastoris is widely used in the manufacture of industrial
enzymes and pharmaceuticals. Like most biotechnological production hosts, P. pastoris is …

Carbon dioxide (CO 2) occurs naturally in the atmosphere as a trace gas, which is produced
naturally as well as by anthropogenic activities. CO 2 is a readily available source of carbon …

Numerous metabolic engineering strategies have allowed yeasts to efficiently assimilate
xylose, the second most abundant sugar component of lignocellulosic biomass. During the …

Technological and medical advances over the past few decades epitomize human
capabilities. However, the increased life expectancies and concomitant land-use changes …

Lignocellulosic biofuel has been globally recognized as a renewable energy source with
environmental benefits while minimizing the competition between food and fuel supply …

This review summarizes progress in the construction of efficient yeast ethanol producers
from glucose/sucrose and lignocellulose. Saccharomyces cerevisiae is the major industrial …

Highlights•Different xylose utilization pathways recently re-constructed in E. coli or S.
cerevisiae are reviewed.•Circumventing metabolic restriction in cells to increase xylose …

Highlights•Metabolic engineering improves microbial production of cellulosic fuels.•We
highlight a paradigm shift in engineering of ethanol producer, S. cerevisiae.•Strain …