Ten years of lateral flow immunoassay technique applications: Trends, challenges and future perspectives

F Di Nardo, M Chiarello, S Cavalera, C Baggiani… - Sensors, 2021 - mdpi.com
The Lateral Flow Immunoassay (LFIA) is by far one of the most successful analytical
platforms to perform the on-site detection of target substances. LFIA can be considered as a …

Huanglongbing pandemic: current challenges and emerging management strategies

D Ghosh, S Kokane, BK Savita, P Kumar, AK Sharma… - Plants, 2022 - mdpi.com
Huanglongbing (HLB, aka citrus greening), one of the most devastating diseases of citrus,
has wreaked havoc on the global citrus industry in recent decades. The culprit behind such …

Recombinase polymerase amplification in the molecular diagnosis of microbiological targets and its applications

DS Mota, JM Guimarães… - Canadian Journal of …, 2022 - cdnsciencepub.com
Since the introduction of the polymerase chain reaction (PCR) technique in 1983, nucleic
acid amplification has permeated all fields of biological science, particularly clinical …

Early molecular markers for retrospective biodosimetry and prediction of acute health effects

M Abend, WF Blakely, P Ostheim… - Journal of …, 2022 - iopscience.iop.org
Radiation-induced biological changes occurring within hours and days after irradiation can
be potentially used for either exposure reconstruction (retrospective dosimetry) or the …

Recent Uses of Paper Microfluidics in Isothermal Nucleic Acid Amplification Tests

J Reynolds, RS Loeffler, PJ Leigh, HA Lopez, JY Yoon - Biosensors, 2023 - mdpi.com
Isothermal nucleic acid amplification tests have recently gained popularity over polymerase
chain reaction (PCR), as they only require a constant temperature and significantly simplify …

The potential use of isothermal amplification assays for in-field diagnostics of plant pathogens

AV Ivanov, IV Safenkova, AV Zherdev, BB Dzantiev - Plants, 2021 - mdpi.com
Rapid, sensitive, and timely diagnostics are essential for protecting plants from pathogens.
Commonly, PCR techniques are used in laboratories for highly sensitive detection of …

CRISPR Diagnostics for Quantification and Rapid Diagnosis of Myotonic Dystrophy Type 1 Repeat Expansion Disorders

K Asano, K Yoshimi, K Takeshita… - ACS Synthetic …, 2024 - ACS Publications
Repeat expansion disorders, exemplified by myotonic dystrophy type 1 (DM1), present
challenges in diagnostic quantification because of the variability and complexity of repeat …

Simple template-based reverse transcription-recombinase polymerase amplification assay for routine diagnosis of citrus tristeza virus

SK Sharma, N Pathaw, B Wangkhem… - Letters in Applied …, 2023 - academic.oup.com
This study reports a simple template-based reverse transcription-polymerase amplification
assay (ST-RT-RPA) for detection of citrus tristeza virus (CTV) from crude plant extract lysed …

RT-RPA and RPA-LFA assay for rapid and ultrasensitive detection of Vibrio parahaemolyticus

A Hu, H Chen, C Shi, Z Lu, F Meng, X Bie - Food Control, 2024 - Elsevier
RPA technology has the advantages of short experimental period, simple operation, and low
equipment requirements, and can be applied to rapid detection in the field of food safety …

Visual detection of porcine epidemic diarrhea virus by recombinase polymerase amplification combined with lateral flow dipstrip

L Ma, K Lian, M Zhu, Y Tang, M Zhang - BMC Veterinary Research, 2022 - Springer
Background Porcine epidemic diarrhea virus (PEDV) is one of the most important enteric
viruses causing diarrhea in pigs. The establishment of a rapid detection method applicable …