Removal of introns from messenger RNA precursors (pre-mRNA splicing) is an essential
step for the expression of most eukaryotic genes. Alternative splicing enables the regulated …

RNA-binding proteins (RBPs) are typically thought of as proteins that bind RNA through one
or multiple globular RNA-binding domains (RBDs) and change the fate or function of the …

Hotspot mutations in splicing factor genes have been recently reported at high frequency in
hematological malignancies, suggesting the importance of RNA splicing in cancer. We …

Next-generation sequencing has revolutionized clinical diagnostic testing. Yet, for a
substantial proportion of patients, sequence information restricted to exons and exon–intron …

Splice-switching oligonucleotides (SSOs) are short, synthetic, antisense, modified nucleic
acids that base-pair with a pre-mRNA and disrupt the normal splicing repertoire of the …

A decline of skeletal muscle strength with aging is a primary cause of mobility loss and frailty
in older persons, but the molecular mechanisms of such decline are not understood. Here …

Serine and arginine-rich (SR) proteins are RNA-binding proteins (RBPs) known as
constitutive and alternative splicing regulators. As splicing is linked to transcriptional and …

The highly intronic nature of protein coding genes in mammals necessitates a co-
transcriptional splicing mechanism as revealed by mNET-seq analysis. Immunoprecipitation …

A large number of mutations in genes that encode RNA binding proteins cause human
disease. Many of these RNA binding proteins mediate key steps in post-transcriptional …

Little is known about the spliceosome's structure before its extensive remodeling into a
catalytically active complex. Here, we report a 3D cryo-EM structure of a pre-catalytic human …