[HTML][HTML] Nanopore sequencing and its application to the study of microbial communities

L Ciuffreda, H Rodríguez-Pérez, C Flores - Computational and structural …, 2021 - Elsevier
Since its introduction, nanopore sequencing has enhanced our ability to study complex
microbial samples through the possibility to sequence long reads in real time using …

Direct RNA sequencing reveals m6A modifications on adenovirus RNA are necessary for efficient splicing

AM Price, KE Hayer, ABR McIntyre, NS Gokhale… - Nature …, 2020 - nature.com
Adenovirus is a nuclear replicating DNA virus reliant on host RNA processing machinery.
Processing and metabolism of cellular RNAs can be regulated by METTL3, which catalyzes …

Nanopore-based detection of viral RNA modifications

JS Abebe, R Verstraten, DP Depledge - Mbio, 2022 - Am Soc Microbiol
The chemical modification of ribonucleotides plays an integral role in the biology of diverse
viruses and their eukaryotic host cells. Mapping the precise identity, location, and …

Adenovirus prevents dsRNA formation by promoting efficient splicing of viral RNA

AM Price, RT Steinbock, C Di, KE Hayer… - Nucleic acids …, 2022 - academic.oup.com
Eukaryotic cells recognize intracellular pathogens through pattern recognition receptors,
including sensors of aberrant nucleic acid structures. Sensors of double-stranded RNA …

Adenovirus remodeling of the host proteome and host factors associated with viral genomes

JM Dybas, KK Lum, K Kulej, ED Reyes, R Lauman… - Msystems, 2021 - Am Soc Microbiol
Viral infections are associated with extensive remodeling of the cellular proteome. Viruses
encode gene products that manipulate host proteins to redirect cellular processes or subvert …

Combined nanopore and single-molecule real-time sequencing survey of human betaherpesvirus 5 transcriptome

B Kakuk, D Tombácz, Z Balázs, N Moldován… - Scientific Reports, 2021 - nature.com
Abstract Long-read sequencing (LRS), a powerful novel approach, is able to read full-length
transcripts and confers a major advantage over the earlier gold standard short-read …

Using direct RNA nanopore sequencing to deconvolute viral transcriptomes

DP Depledge, AC Wilson - Current protocols in microbiology, 2020 - Wiley Online Library
The genomes of DNA viruses encode deceptively complex transcriptomes evolved to
maximize coding potential within the confines of a relatively small genome. Defining the full …