Towards application of CRISPR-Cas12a in the design of modern viral DNA detection tools

J Dronina, U Samukaite-Bubniene… - Journal of …, 2022 - Springer
Early detection of viral pathogens by DNA-sensors in clinical samples, contaminated foods,
soil or water can dramatically improve clinical outcomes and reduce the socioeconomic …

CRISPR genetic toolkits of classical food microorganisms: Current state and future prospects

X Lv, Y Li, X Xiu, C Liao, Y Xu, Y Liu, J Li, G Du… - Biotechnology …, 2023 - Elsevier
Production of food-related products using microorganisms in an environmentally friendly
manner is a crucial solution to global food safety and environmental pollution issues …

CRISPR-assisted rational flux-tuning and arrayed CRISPRi screening of an l-proline exporter for l-proline hyperproduction

J Liu, M Liu, T Shi, G Sun, N Gao, X Zhao… - Nature …, 2022 - nature.com
Abstract Development of hyperproducing strains is important for biomanufacturing of
biochemicals and biofuels but requires extensive efforts to engineer cellular metabolism and …

Large chromosomal segment deletions by CRISPR/LbCpf1‐mediated multiplex gene editing in soybean

K Duan, Y Cheng, J Ji, C Wang, Y Wei… - Journal of Integrative …, 2021 - Wiley Online Library
The creation of new soybean varieties has been limited by genomic duplication and
redundancy. Efficient multiplex gene editing and large chromosomal segment deletion …

Advances and Perspectives for Genome Editing Tools of Corynebacterium glutamicum

Q Wang, J Zhang, NH Al Makishah, X Sun… - Frontiers in …, 2021 - frontiersin.org
Corynebacterium glutamicum has been considered a promising synthetic biological platform
for biomanufacturing and bioremediation. However, there are still some challenges in …

[HTML][HTML] Synthetic biology tools for engineering Corynebacterium glutamicum

GY Kim, J Kim, G Park, HJ Kim, J Yang… - Computational and …, 2023 - Elsevier
Corynebacterium glutamicum is a promising organism for the industrial production of amino
acids, fuels, and various value-added chemicals. From the whole genome sequence …

Recent advances of Cas12a applications in bacteria

M Meliawati, C Schilling, J Schmid - Applied Microbiology and …, 2021 - Springer
Clustered regularly interspaced short palindromic repeats (CRISPR)-mediated genome
engineering and related technologies have revolutionized biotechnology over the last …

[HTML][HTML] CRISPR-based gene editing technology and its application in microbial engineering

J Wei, Y Li - Engineering Microbiology, 2023 - Elsevier
Gene editing technology involves the modification of a specific target gene to obtain a new
function or phenotype. Recent advances in clustered regularly interspaced short palindromic …

Enhanced production of d-pantothenic acid in Corynebacterium glutamicum using an efficient CRISPR–Cpf1 genome editing method

R Su, T Wang, T Bo, N Cai, M Yuan, C Wu… - Microbial Cell …, 2023 - Springer
Background Corynebacterium glutamicum has industrial track records for producing a
variety of valuable products such as amino acids. Although CRISPR-based genome editing …

The application of CRISPR/Cas mediated gene editing in synthetic biology: Challenges and optimizations

W Li, C Huang, J Chen - Frontiers in Bioengineering and …, 2022 - frontiersin.org
Clustered regularly interspaced short palindromic repeats (CRISPR) and its associated
enzymes (Cas) is a simple and convenient genome editing tool that has been used in …