Firefly luciferase-catalyzed reaction proceeds via the initial formation of an enzyme-bound
luciferyl adenylate intermediate. The chemical origin of the color modulation in firefly …

The color of firefly bioluminescence is determined by the structure of luciferase. Firefly
luciferase genes have been isolated from more than 30 species, producing light ranging in …

Firefly luciferase is widely used in a number of areas of biotechnology and molecular
biology. However, rapid inactivation of wild-type (WT) luciferases at elevated temperatures …

Despite of growing interest in use of carbon-based nanomaterials as carriers of functional
proteins, less attention has been paid to the effects of these nanomaterials on the structure …

Background Nanoparticles have emerged as key materials for developing applications in
nanomedicine, nanobiotechnology, bioimaging and theranostics. Existing bioimaging …

In most bioluminescence systems the oxidation of luciferin and production of light is
catalyzed by luciferases. Protein engineering studies have shown that thermostable proteins …

This paper investigates the impact of taurine as an additive on the structural and functional
stability of urate oxidase. First, the effect of the processing parameters for the stabilization of …

Although synonymous mutations have long been thought to lack striking results, a growing
body of research shows these mutations have highly variable effects. In this study, the …

Nanobiocatalysis is a novel area integrating various advantages of nanotechnology and
enzymatic catalysis. However, great efforts are still needed to fully understand the …

To date, two Iranian luciferase genes from the Lampyris turkestanicus and Lampyroidea
maculata have been carefully studied. Here, we report the cloning and characterization of …