A guide to metabolic flux analysis in metabolic engineering: Methods, tools and applications
MR Antoniewicz - Metabolic engineering, 2021 - Elsevier
The field of metabolic engineering is primarily concerned with improving the biological
production of value-added chemicals, fuels and pharmaceuticals through the design …
production of value-added chemicals, fuels and pharmaceuticals through the design …
Understanding metabolism with flux analysis: From theory to application
Z Dai, JW Locasale - Metabolic engineering, 2017 - Elsevier
Quantitative and qualitative knowledge of metabolic rates (ie fluxes) over a metabolic
network and in specific cellular compartments gives insights into the regulation of …
network and in specific cellular compartments gives insights into the regulation of …
High-resolution 13C metabolic flux analysis
CP Long, MR Antoniewicz - Nature protocols, 2019 - nature.com
Precise quantification of metabolic pathway fluxes in biological systems is of major
importance in guiding efforts in metabolic engineering, biotechnology, microbiology, human …
importance in guiding efforts in metabolic engineering, biotechnology, microbiology, human …
Metabolic flux analysis: a comprehensive review on sample preparation, analytical techniques, data analysis, computational modelling, and main application areas
Metabolic flux analysis (MFA) quantitatively describes cellular fluxes to understand
metabolic phenotypes and functional behaviour after environmental and/or genetic …
metabolic phenotypes and functional behaviour after environmental and/or genetic …
Yeast increases glycolytic flux to support higher growth rates accompanied by decreased metabolite regulation and lower protein phosphorylation
M Chen, T Xie, H Li, Y Zhuang… - Proceedings of the …, 2023 - National Acad Sciences
Supply of Gibbs free energy and precursors are vital for cellular function and cell metabolism
have evolved to be tightly regulated to balance their supply and consumption. Precursors …
have evolved to be tightly regulated to balance their supply and consumption. Precursors …
FreeFlux: A Python Package for Time-Efficient Isotopically Nonstationary Metabolic Flux Analysis
13C metabolic flux analysis is a powerful tool for metabolism characterization in metabolic
engineering and synthetic biology. However, the widespread adoption of this tool is …
engineering and synthetic biology. However, the widespread adoption of this tool is …
Amino acid is a major carbon source for hepatic lipogenesis
Y Liao, Q Chen, L Liu, H Huang, J Sun, X Bai, C Jin… - Cell Metabolism, 2024 - cell.com
Increased de novo lipogenesis is a hallmark of metabolic dysfunction-associated steatotic
liver disease (MASLD) in obesity, but the macronutrient carbon source for over half of …
liver disease (MASLD) in obesity, but the macronutrient carbon source for over half of …
Recent advances in constraint and machine learning-based metabolic modeling by leveraging stoichiometric balances, thermodynamic feasibility and kinetic law …
Understanding the governing principles behind organisms' metabolism and growth
underpins their effective deployment as bioproduction chassis. A central objective of …
underpins their effective deployment as bioproduction chassis. A central objective of …
Isotopically nonstationary metabolic flux analysis (INST-MFA): putting theory into practice
Typically, 13 C flux analysis relies on assumptions of both metabolic and isotopic steady
state. If metabolism is steady but isotope labeling is not allowed to fully equilibrate …
state. If metabolism is steady but isotope labeling is not allowed to fully equilibrate …
Systematic identification and elimination of flux bottlenecks in the aldehyde production pathway of Synechococcus elongatus PCC 7942
Isotopically nonstationary metabolic flux analysis (INST-MFA) provides a versatile platform to
quantitatively assess in vivo metabolic activities of autotrophic systems. By applying INST …
quantitatively assess in vivo metabolic activities of autotrophic systems. By applying INST …