Background In recent years, massively parallel complementary DNA sequencing (RNA
sequencing [RNA-Seq]) has emerged as a fast, cost-effective, and robust technology to …

De novo assembly of a complete transcriptome without the need for a guiding reference
genome is attractive, particularly where the cost and complexity of generating a eukaryote …

The sea represents a major source of biodiversity. It exhibits many different ecosystems in a
huge variety of environmental conditions where marine organisms have evolved with …

De novo RNA-Seq assembly facilitates the study of transcriptomes for species without
sequenced genomes, but it is challenging to select the most accurate assembly in this …

Background Salmonid fishes exhibit high levels of phenotypic and ecological variation and
are thus ideal model systems for studying evolutionary processes of adaptive divergence …

Next-generation sequencing–based metagenomics has enabled to identify microorganisms
in characteristic habitats without the need for lengthy cultivation. Importantly, clinically …

Chinese hamster ovary (CHO) cell lines represent the most commonly used mammalian
expression system for the production of therapeutic proteins. In this context, detailed …

Increasing salinity levels in freshwater and coastal environments caused by sea level rise
linked to climate change is now recognized to be a major factor that can impact fish growth …

The large amount of biological data available in the current times, makes it necessary to use
tools and applications based on sophisticated and efficient algorithms, developed in the …

Analysis of RNA-sequence (RNA-seq) data is widely used in transcriptomic studies and it
has many applications. We review RNA-seq data analysis from RNA-seq reads to the results …