Synthetic biology: bottom-up assembly of molecular systems

S Hirschi, TR Ward, WP Meier, DJ Müller… - Chemical …, 2022 - ACS Publications
The bottom-up assembly of biological and chemical components opens exciting
opportunities to engineer artificial vesicular systems for applications with previously unmet …

Osmosis, from molecular insights to large-scale applications

S Marbach, L Bocquet - Chemical Society Reviews, 2019 - pubs.rsc.org
Osmosis is a universal phenomenon occurring in a broad variety of processes and fields. It
is the archetype of entropic forces, both trivial in its fundamental expression–the van't Hoff …

[HTML][HTML] In vitro assembly, positioning and contraction of a division ring in minimal cells

S Kohyama, A Merino-Salomón, P Schwille - Nature Communications, 2022 - nature.com
Constructing a minimal machinery for autonomous self-division of synthetic cells is a major
goal of bottom-up synthetic biology. One paradigm has been the E. coli divisome, with the …

From the regulation of peptidoglycan synthesis to bacterial growth and morphology

A Typas, M Banzhaf, CA Gross… - Nature Reviews …, 2012 - nature.com
How bacteria grow and divide while retaining a defined shape is a fundamental question in
microbiology, but technological advances are now driving a new understanding of how the …

[HTML][HTML] Octanol-assisted liposome assembly on chip

S Deshpande, Y Caspi, AEC Meijering… - Nature …, 2016 - nature.com
Liposomes are versatile supramolecular assemblies widely used in basic and applied
sciences. Here we present a novel microfluidics-based method, octanol-assisted liposome …

Actin, a central player in cell shape and movement

TD Pollard, JA Cooper - science, 2009 - science.org
The protein actin forms filaments that provide cells with mechanical support and driving
forces for movement. Actin contributes to biological processes such as sensing …

Splitsville: structural and functional insights into the dynamic bacterial Z ring

DP Haeusser, W Margolin - Nature Reviews Microbiology, 2016 - nature.com
Bacteria must divide to increase in number and colonize their niche. Binary fission is the
most widespread means of bacterial cell division, but even this relatively simple mechanism …

Microfluidic assembly of monodisperse vesosomes as artificial cell models

NN Deng, M Yelleswarapu, L Zheng… - Journal of the American …, 2017 - ACS Publications
Vesosomes are nested liposomal structures with high potential as advanced drug delivery
vehicles, bioreactors and artificial cells. However, to date no method has been reported to …

The bacterial cell division proteins FtsA and FtsZ self-organize into dynamic cytoskeletal patterns

M Loose, TJ Mitchison - Nature cell biology, 2014 - nature.com
Bacterial cytokinesis is commonly initiated by the Z-ring, a cytoskeletal structure that
assembles at the site of division. Its primary component is FtsZ, a tubulin superfamily …

[HTML][HTML] Gel-assisted formation of giant unilamellar vesicles

A Weinberger, FC Tsai, GH Koenderink, TF Schmidt… - Biophysical journal, 2013 - cell.com
Giant unilamellar vesicles or GUVs are systems of choice as biomimetic models of cellular
membranes. Although a variety of procedures exist for making single walled vesicles of tens …