Recombination-mediated genetic engineering, also known as recombineering, is the
genomic incorporation of homologous single-stranded or double-stranded DNA into …

The bacterial chromosome and bacterial plasmids can be engineered in vivo by
homologous recombination using PCR products and synthetic oligonucleotides as …

Critical cancer pathways often cannot be targeted because of limited efficiency crossing cell
membranes. Here we report the development of a Salmonella-based intracellular delivery …

Engineering cellular metabolism for improved production of valuable chemicals requires
extensive modulation of bacterial genome to explore complex genetic spaces. Here, we …

We describe the construction and characterization of a genomically recoded organism
(GRO). We replaced all known UAG stop codons in Escherichia coli MG1655 with …

The assembly of DNA fragments with homologous arms is becoming popular in routine
cloning. For an in vitro assembly reaction, a DNA polymerase is often used either alone for …

Functional analysis of genome sequences requires methods for cloning DNA of interest.
However, existing methods, such as library cloning and screening, are too demanding or …

Genome engineering methods in E. coli allow for easy to perform manipulations of the
chromosome in vivo with the assistance of the λ-Red recombinase system. These methods …

A bottleneck in metabolic engineering and systems biology approaches is the lack of
efficient genome engineering technologies. Here, we combine CRISPR/Cas9 and λ Red …

To date, most genetic engineering approaches coupling the type II Streptococcus pyogenes
clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system to lambda …