Apoptotic detection methods—from morphology to gene
Y Otsuki, Z Li, MA Shibata - Progress in histochemistry and cytochemistry, 2003 - Elsevier
To date, many terms have been given for cell death and forgotten before they became
widely accepted. Most researchers studying cell death use some terms for cell death that …
widely accepted. Most researchers studying cell death use some terms for cell death that …
Single-cell gene expression profiling using reverse transcription quantitative real-time PCR
A Ståhlberg, M Bengtsson - Methods, 2010 - Elsevier
Even in an apparently homogeneous population of cells there are considerable differences
between individual cells. A response to a stimulus of a cell population or tissue may be …
between individual cells. A response to a stimulus of a cell population or tissue may be …
Identification and characterization of immunoglobulin G in blood as a major inhibitor of diagnostic PCR
WA Al-Soud, LJ Jönsson… - Journal of clinical …, 2000 - Am Soc Microbiol
ABSTRACT A major inhibitor of diagnostic PCR in human plasma was identified and the
mechanism of inhibition was characterized. Human blood was divided by centrifugation into …
mechanism of inhibition was characterized. Human blood was divided by centrifugation into …
Evaluation of digital PCR for absolute RNA quantification
Gene expression measurements detailing mRNA quantities are widely employed in
molecular biology and are increasingly important in diagnostic fields. Reverse transcription …
molecular biology and are increasingly important in diagnostic fields. Reverse transcription …
Development of a real-time RT-PCR assay for the detection of Potato spindle tuber viroid
N Boonham, LG Pérez, MS Mendez, EL Peralta… - Journal of virological …, 2004 - Elsevier
Potato spindle tuber viroid (PSTVd) is a quarantine pathogen in the European Union and
causes damaging diseases of solanaceous crops. Under the EU Plant Health directive …
causes damaging diseases of solanaceous crops. Under the EU Plant Health directive …
Quantification of mRNA in single cells and modelling of RT-qPCR induced noise
Background Gene expression has a strong stochastic element resulting in highly variable
mRNA levels between individual cells, even in a seemingly homogeneous cell population …
mRNA levels between individual cells, even in a seemingly homogeneous cell population …
Sensitive detection and typing of porcine reproductive and respiratory syndrome virus by RT-PCR amplification of whole viral genes
MB Oleksiewicz, A Bøtner, KG Madsen… - Veterinary microbiology, 1998 - Elsevier
Following the recent use of a live vaccine against porcine reproductive and respiratory
syndrome virus (PRRSV) in Denmark, both American (vaccine) and European-type PRRSV …
syndrome virus (PRRSV) in Denmark, both American (vaccine) and European-type PRRSV …
PCR inhibition by reverse transcriptase leads to an overestimation of amplification efficiency
O Suslov, DA Steindler - Nucleic acids research, 2005 - academic.oup.com
This study addresses the problem of PCR inhibition by reverse transcriptase. It has been
shown that the inhibition occurs mostly when a small amount of RNA is taken for RT …
shown that the inhibition occurs mostly when a small amount of RNA is taken for RT …
Reverse transcriptase (RT) inhibition of PCR at low concentrations of template and its implications for quantitative RT-PCR
DP Chandler, CA Wagnon… - Applied and …, 1998 - Am Soc Microbiol
Numerous instances of reverse transcriptase (RT) inhibition of the PCR were observed while
developing nonquantitative uncoupled RT-PCR techniques for detecting nitrogenase and …
developing nonquantitative uncoupled RT-PCR techniques for detecting nitrogenase and …
Effects of single-walled carbon nanotubes on the polymerase chain reaction
The effects of single-walled carbon nanotubes on the polymerase chain reaction (PCR)
were investigated via quantitative PCR product measurements, scanning electron …
were investigated via quantitative PCR product measurements, scanning electron …