Applications of the Saccharomyces cerevisiae Flp-FRT System in Bacterial Genetics

HP Schweizer - Journal of molecular microbiology and biotechnology, 2003 - karger.com
The Flp-FRT site-specific recombination system from Saccharomyces cerevisiae is a
powerful and efficient tool for high-throughput genetic analysis of bacteria in the …

Painting with light-powered bacteria

J Arlt, VA Martinez, A Dawson, T Pilizota… - Nature …, 2018 - nature.com
Self-assembly is a promising route for micro-and nano-fabrication with potential to
revolutionise many areas of technology, including personalised medicine. Here we …

Engineering AraC to make it responsive to light instead of arabinose

E Romano, A Baumschlager, EB Akmeric… - Nature chemical …, 2021 - nature.com
The l-arabinose-responsive AraC and its cognate PBAD promoter underlie one of the most
often used chemically inducible prokaryotic gene expression systems in microbiology and …

Why Is Carbonic Anhydrase Essential to Escherichia coli?

C Merlin, M Masters, S McAteer… - Journal of bacteriology, 2003 - Am Soc Microbiol
The can (previously yadF) gene of Escherichia coli encodes a β-class carbonic anhydrase
(CA), an enzyme which interconverts CO2 and bicarbonate. Various essential metabolic …

Proteomic and microarray characterization of the AggR regulon identifies a pheU pathogenicity island in enteroaggregative Escherichia coli

EG Dudley, NR Thomson, J Parkhill… - Molecular …, 2006 - Wiley Online Library
Enteroaggregative Escherichia coli (EAEC) is defined by aggregative adherence (AA) to
HEp‐2 cells, where bacteria display adherence to cell surfaces and also to the intervening …

Swimming in a crystal

AT Brown, ID Vladescu, A Dawson, T Vissers… - Soft matter, 2016 - pubs.rsc.org
We study catalytic Janus particles and Escherichia coli bacteria swimming in a two-
dimensional colloidal crystal. The Janus particles orbit individual colloids and hop between …

The life-cycle of operons

MN Price, AP Arkin, EJ Alm - PLoS genetics, 2006 - journals.plos.org
Operons are a major feature of all prokaryotic genomes, but how and why operon structures
vary is not well understood. To elucidate the life-cycle of operons, we compared gene order …

Modulators of a robust and efficient metabolism: perspective and insights from the Rid superfamily of proteins

RL Fulton, DM Downs - Advances in microbial physiology, 2023 - Elsevier
Metabolism is an integrated network of biochemical pathways that assemble to generate the
robust, responsive physiologies of microorganisms. Despite decades of fundamental studies …

Characterization of RarA, a novel AraC family multidrug resistance regulator in Klebsiella pneumoniae

M Veleba, PG Higgins, G Gonzalez… - Antimicrobial agents …, 2012 - Am Soc Microbiol
Transcriptional regulators, such as SoxS, RamA, MarA, and Rob, which upregulate the
AcrAB efflux pump, have been shown to be associated with multidrug resistance in clinically …

Run-to-Tumble Variability Controls the Surface Residence Times of E. coli Bacteria

G Junot, T Darnige, A Lindner, VA Martinez, J Arlt… - Physical Review Letters, 2022 - APS
Motile bacteria are known to accumulate at surfaces, eventually leading to changes in
bacterial motility and biofilm formation. We use a novel two-color, three-dimensional …