Establishment of H3K9-methylated heterochromatin and its functions in tissue differentiation and maintenance

J Padeken, SP Methot, SM Gasser - Nature Reviews Molecular Cell …, 2022 - nature.com
Heterochromatin is characterized by dimethylated or trimethylated histone H3 Lys9
(H3K9me2 or H3K9me3, respectively) and is found at transposable elements, satellite …

The molecular basis of heterochromatin assembly and epigenetic inheritance

SIS Grewal - Molecular cell, 2023 - cell.com
Heterochromatin plays a fundamental role in gene regulation, genome integrity, and
silencing of repetitive DNA elements. Histone modifications are essential for the …

[HTML][HTML] Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing

JK Nuñez, J Chen, GC Pommier, JZ Cogan… - Cell, 2021 - cell.com
A general approach for heritably altering gene expression has the potential to enable many
discovery and therapeutic efforts. Here, we present CRISPRoff—a programmable epigenetic …

Reevaluating the roles of histone-modifying enzymes and their associated chromatin modifications in transcriptional regulation

MAJ Morgan, A Shilatifard - Nature genetics, 2020 - nature.com
Histone-modifying enzymes are implicated in the control of diverse DNA-templated
processes including gene expression. Here, we outline historical and current thinking …

Automethylation of PRC2 promotes H3K27 methylation and is impaired in H3K27M pediatric glioma

CH Lee, JR Yu, J Granat, R Saldaña-Meyer… - Genes & …, 2019 - genesdev.cshlp.org
The histone methyltransferase activity of PRC2 is central to the formation of H3K27me3-
decorated facultative heterochromatin and gene silencing. In addition, PRC2 has been …

[HTML][HTML] Regulatory mechanisms governing chromatin organization and function

R Villaseñor, T Baubec - Current Opinion in Cell Biology, 2021 - Elsevier
Nucleosomes, the basic structures used to package genetic information into chromatin, are
subject to a diverse array of chemical modifications. A large number of these marks serve as …

Drug design targeting active posttranslational modification protein isoforms

F Meng, Z Liang, K Zhao, C Luo - Medicinal research reviews, 2021 - Wiley Online Library
Modern drug design aims to discover novel lead compounds with attractable chemical
profiles to enable further exploration of the intersection of chemical space and biological …

Caenorhabditis elegans nuclear RNAi factor SET-32 deposits the transgenerational histone modification, H3K23me3

L Schwartz-Orbach, C Zhang, S Sidoli, R Amin, D Kaur… - Elife, 2020 - elifesciences.org
Nuclear RNAi provides a highly tractable system to study RNA-mediated chromatin changes
and epigenetic inheritance. Recent studies have indicated that the regulation and function of …

Approaching the catalytic mechanism of protein lysine methyltransferases by biochemical and simulation techniques

P Schnee, J Pleiss, A Jeltsch - Critical Reviews in Biochemistry and …, 2024 - Taylor & Francis
Protein lysine methyltransferases (PKMTs) transfer up to three methyl groups to the side
chains of lysine residues in proteins and fulfill important regulatory functions by controlling …

Uncoupling the distinct functions of HP1 proteins during heterochromatin establishment and maintenance

M Seman, A Levashkevich, A Larkin, F Huang… - Cell reports, 2023 - cell.com
Summary H3K9 methylation (H3K9me) marks transcriptionally silent genomic regions called
heterochromatin. HP1 proteins are required to establish and maintain heterochromatin. HP1 …