The method of displaying recombinant proteins on the surface of Saccharomyces cerevisiae
via genetic fusion to an abundant cell wall protein, a technology known as yeast surface …

Since its first application to antibody engineering 15years ago, yeast display technology has
been developed into a highly potent tool for both affinity maturing lead molecules and …

Molecular ON-switches in which a chemical compound induces protein–protein interactions
can allow cellular function to be controlled with small molecules. ON-switches based on …

The demand for recombinant therapeutic antibodies and Fc-fusion proteins is expected to
increase in the years to come. Hence, extensive efforts are concentrated on improving the …

Binding proteins are typically isolated from combinatorial libraries of scaffold proteins using
one of the many library screening tools available, such as phage display, yeast surface …

In recent years, several cell-based screening technologies for the isolation of antibodies with
prescribed properties emerged. They rely on the multi-copy display of antibodies or antibody …

Pioneered exactly 20 years ago, yeast surface display (YSD) continues to take a major role
in protein engineering among the high-throughput display methodologies that have been …

Yeast surface display is a powerful technology for engineering a broad range of protein
scaffolds. This protocol describes the process for de novo isolation of protein binders from …

In addition to its biological function, the stability of a protein is a major determinant for its
applicability. Unfortunately, engineering proteins for improved functionality usually results in …

Small protein ligands can provide superior physiological distribution compared with
antibodies, and improved stability, production, and specific conjugation. Systematic …