Abstract The type VF CRISPR-Cas12f system is a strong candidate for therapeutic
applications due to the compact size of the Cas12f proteins. In this work, we identify six …

Genome editing technologies hold great promise for numerous applications including the
understanding of cellular and disease mechanisms and the development of gene and …

The rapid growth of global biopharmaceutical market in the recent years has been a good
indication of its significance in biotechnology industry. During a long period of time in …

The clustered regularly interspaced short palindromic repeats-Cas (CRISPR-Cas) systems,
including type II Cas9 and type V Cas12 systems, which serve in the adaptive immunity of …

Summary The type VI CRISPR-Cas system is becoming increasingly more attractive for
genome editing. However, natural nucleases of this system often exhibit low efficiency …

Transposon-associated ribonucleoprotein TnpB is known to be the ancestry endonuclease
of diverse Cas12 effector proteins from type-V CRISPR system. Given its small size (408 aa) …

Background Ovarian cancer stem cells (OCSCs) contribute to the poor prognosis of ovarian
cancer. Involvement of the androgen receptor (AR) in the malignant behaviors of other …

In brief IFN-λs participate in the fetal–maternal immune interaction, involving in immune
regulation, uterine receptivity, cell migration and adhesion, and endometrium apoptosis. Our …

CRISPR–Cas9 is a powerful tool for genome engineering, but its efficiency largely depends
on guide RNA (gRNA). There are multiple methods available to evaluate the efficiency of …

Prime editor, an editing tool based on the CRISPR/Cas9 system, allows for all 12 types of
nucleotide exchanges and arbitrary indels in genomic sequences without the need for …