Despite its short history, diffraction-unlimited fluorescence microscopy techniques have
already made a substantial imprint in the biological sciences. In this review, we describe …

Biofuel cells are energy conversion devices that use biocatalysts to convert chemical energy
to electrical energy. Over the last decade, research in this area has intensified, especially in …

The interaction of lipids and proteins plays an important role in plasma membrane
bioactivity, and much can be learned from their diffusion characteristics. Here we present the …

This protocol outlines a procedure for collecting and analyzing point spread functions
(PSFs). It describes how to prepare fluorescent microsphere samples, set up a confocal …

Single-point fluorescence correlation spectroscopy (FCS) allows measurements of fast
diffusion and dynamic processes in the microsecond-to-millisecond time range. For …

Highly sensitive fluorescence microscopy techniques allow single nanoparticles to be
tracked during their uptake into living cells with high temporal and spatial resolution. From …

Investigation of lipid lateral mobility in biological membranes and their artificial models
provides information on membrane dynamics and structure; methods based on optical …

Anomalous subdiffusion has been reported for two-dimensional diffusion in the plasma
membrane and three-dimensional diffusion in the nucleus and cytoplasm. If a particle …

In the recent decade, single-molecule (sm) spectroscopy has come of age and is providing
important insight into how biological molecules function. So far our view of protein function is …

For a little more than a century, fluorescence microscopy has been an essential source of
major discoveries in cell biology. Recent developments improved both visualization and …