Discovery of diverse CRISPR-Cas systems and expansion of the genome engineering toolbox
CRISPR systems mediate adaptive immunity in bacteria and archaea through diverse
effector mechanisms and have been repurposed for versatile applications in therapeutics …
effector mechanisms and have been repurposed for versatile applications in therapeutics …
Sequence modeling and design from molecular to genome scale with Evo
The genome is a sequence that encodes the DNA, RNA, and proteins that orchestrate an
organism's function. We present Evo, a long-context genomic foundation model with a …
organism's function. We present Evo, a long-context genomic foundation model with a …
Prediction of on-target and off-target activity of CRISPR–Cas13d guide RNAs using deep learning
HH Wessels, A Stirn, A Méndez-Mancilla, EJ Kim… - Nature …, 2024 - nature.com
Transcriptome engineering applications in living cells with RNA-targeting CRISPR effectors
depend on accurate prediction of on-target activity and off-target avoidance. Here we design …
depend on accurate prediction of on-target activity and off-target avoidance. Here we design …
A versatile CRISPR-Cas13d platform for multiplexed transcriptomic regulation and metabolic engineering in primary human T cells
CRISPR technologies have begun to revolutionize T cell therapies; however, conventional
CRISPR-Cas9 genome-editing tools are limited in their safety, efficacy, and scope. To …
CRISPR-Cas9 genome-editing tools are limited in their safety, efficacy, and scope. To …
[HTML][HTML] Approaches to probe and perturb long noncoding RNA functions in diseases
Long noncoding RNAs (lncRNAs) are a class of RNA molecules exceeding 200 nucleotides
in length that lack long open-reading frames. Transcribed predominantly by RNA …
in length that lack long open-reading frames. Transcribed predominantly by RNA …
Efficient combinatorial targeting of RNA transcripts in single cells with Cas13 RNA Perturb-seq
Pooled CRISPR screens coupled with single-cell RNA-sequencing have enabled systematic
interrogation of gene function and regulatory networks. Here, we introduce Cas13 RNA …
interrogation of gene function and regulatory networks. Here, we introduce Cas13 RNA …
CRISPR-dCas13d-based deep screening of proximal and distal splicing-regulatory elements
Y Recinos, D Ustianenko, YT Yeh, X Wang… - Nature …, 2024 - nature.com
Pre-mRNA splicing, a key process in gene expression, can be therapeutically modulated
using various drug modalities, including antisense oligonucleotides (ASOs). However …
using various drug modalities, including antisense oligonucleotides (ASOs). However …
Programmed RNA editing with an evolved bacterial adenosine deaminase
Programmed RNA editing presents an attractive therapeutic strategy for genetic disease. In
this study, we developed bacterial deaminase-enabled recoding of RNA (DECOR), which …
this study, we developed bacterial deaminase-enabled recoding of RNA (DECOR), which …
Modeling CRISPR-Cas13d on-target and off-target effects using machine learning approaches
A major challenge in the application of the CRISPR-Cas13d system is to accurately predict
its guide-dependent on-target and off-target effect. Here, we perform CRISPR-Cas13d …
its guide-dependent on-target and off-target effect. Here, we perform CRISPR-Cas13d …
Repurposing CRISPR-Cas13 systems for robust mRNA trans-splicing
DN Fiflis, NA Rey, H Venugopal-Lavanya… - Nature …, 2024 - nature.com
Type VI CRISPR enzymes have been developed as programmable RNA-guided Cas
proteins for eukaryotic RNA editing. Notably, Cas13 has been utilized for site-targeted single …
proteins for eukaryotic RNA editing. Notably, Cas13 has been utilized for site-targeted single …