The last few years have witnessed an unprecedented increase in the number of novel
bacterial species that hold potential to be used for metabolic engineering. Historically …

Bacteria often encounter temperature fluctuations in their natural habitats and must adapt to
survive. The molecular response of bacteria to sudden temperature upshift or downshift is …

Antibacterial agents target the products of essential genes but rarely achieve complete
target inhibition. Thus, the all-or-none definition of essentiality afforded by traditional genetic …

Bacillus subtilis is a Gram-positive model bacterium with extensive documented annotation.
However, with the rise of high-throughput techniques, the amount of complex data being …

Addressing the ongoing antibiotic crisis requires the discovery of compounds with novel
mechanisms of action that are capable of treating drug-resistant infections. Many antibiotics …

Gene essentiality is a founding concept of genetics with important implications in both
fundamental and applied research. Multiple screens have been performed over the years in …

Bacillus subtilis is the most characterized gram-positive bacterium that has significant
attributes, such as growing well on cheap carbon sources, possessing clear inherited …

Living cells are made up of individual parts, ie the genome, the proteins, the RNA and lipid
molecules as well as the metabolites and ions. However, life depends on the functional …

The vast majority of bacteria, including human pathogens and microbiome species, lack
genetic tools needed to systematically associate genes with phenotypes. This is the major …

To fully exploit the microbial genome resources, a high-throughput experimental platform is
needed to associate genes with phenotypes at the genome level. We present here a novel …