Impressive though the achievements of single-particle cryo–electron microscopy are today,
a substantial gap still remains between what is currently accomplished and what is …

After gradually moving away from preparation methods prone to artefacts such as plastic
embedding and negative staining for cell sections and single particles, the field of cryo …

In a surprisingly short time, electron cryo-microscopy (cryoEM) has developed from a niche
technique in structural biology to a mainstream method practiced in a rapidly growing …

First results on the experimental realisation of a 2× 2 programmable phase plate for
electrons are presented. The design consists of an array of electrostatic elements that …

In this paper, we review the current state of phase plate imaging in a transmission electron
microscope. We focus especially on the hole-free phase plate design, also referred to as the …

In this overview, we briefly outline recent advances in electron cryomicroscopy (cryoEM) and
explain why the journal IUCrJ, published by the International Union of Crystallography …

A current bottleneck in structure determination of macromolecular complexes by cryo
electron microscopy (cryo-EM) is the large amount of data needed to obtain high-resolution …

A Boersch phase plate can shift the phase of electrons proportionally to the applied
electrical potential, thereby allowing for in situ control of the electron phase shift. A device …

Structural analysis of macromolecular assemblies and their remodeling during physiological
processes is instrumental to defining the fundament of cellular and molecular biology …

Cryo electron microscopy (cryo-EM) has become a method of choice in structural biology to
analyze isolated complexes and cellular structures. This implies adequate imaging of the …