Single nucleotide polymorphisms (SNPs) are associated with many human diseases, so
accurate and efficient SNP detection is of great significance for early diagnosis and clinical …

Establishing the Kirsten rat sarcoma (KRAS) mutational status is essential in terms of
managing patients with various types of cancer. Allele-specific real-time polymerase chain …

Detection of the Kirsten rat sarcoma gene (KRAS) mutational status is an important factor for
the treatment of various malignancies. The most common KRAS-activating mutations are …

Polymerase chain reaction (PCR) is a powerful molecular biology assay for gene detection
and quantification. Conventional DNA primers for PCR often suffer from poor sensitivity in …

Antimony sulphide thin films have been developed by the dip method. The obtained thin
films were undergone heat treatment up to 473 K in the atmosphere. The variation in …

The poor fidelity of T4 DNA ligase has always limited the simple detection of single-
nucleotide polymorphisms (SNPs) and is only applicable to some special SNP types. This …

Phosphoryl guanidine (PG) is the novel uncharged modification of internucleotide
phosphates of oligonucleotides. Incorporating PG modification into PCR primers leads to …

Abstract Background Phage λ-Red recombineering is a powerful genetic tool to edit the
bacterial genome. With the demand of the multiplex genome editing, the efficiency of …

Antisense oligonucleotides (ASOs) are molecules used to regulate RNA expression by
targeting specific RNA sequences. One specific type of ASO, known as neutralized DNA …

Hybridization probes have been used in the detection of specific nucleic acids for the last 50
years. Despite the extensive efforts and the great significance, the challenges of the …