[HTML][HTML] Universal and high-fidelity DNA single nucleotide polymorphism detection based on a CRISPR/Cas12a biochip
Y Chen, Y Mei, X Jiang - Chemical Science, 2021 - pubs.rsc.org
Single nucleotide polymorphisms (SNPs) are associated with many human diseases, so
accurate and efficient SNP detection is of great significance for early diagnosis and clinical …
accurate and efficient SNP detection is of great significance for early diagnosis and clinical …
[HTML][HTML] Allele-Specific PCR for KRAS Mutation Detection Using Phosphoryl Guanidine Modified Primers
Establishing the Kirsten rat sarcoma (KRAS) mutational status is essential in terms of
managing patients with various types of cancer. Allele-specific real-time polymerase chain …
managing patients with various types of cancer. Allele-specific real-time polymerase chain …
[HTML][HTML] Phosphoramidate Azole Oligonucleotides for Single Nucleotide Polymorphism Detection by PCR
AS Chubarov, EE Baranovskaya, IP Oscorbin… - International Journal of …, 2024 - mdpi.com
Detection of the Kirsten rat sarcoma gene (KRAS) mutational status is an important factor for
the treatment of various malignancies. The most common KRAS-activating mutations are …
the treatment of various malignancies. The most common KRAS-activating mutations are …
[HTML][HTML] Molecular effects of site-specific phosphate-methylated primer on the structure and motions of Taq DNA polymerase
YC Tsai, WY Chen, C Chiu - Computational and Structural Biotechnology …, 2023 - Elsevier
Polymerase chain reaction (PCR) is a powerful molecular biology assay for gene detection
and quantification. Conventional DNA primers for PCR often suffer from poor sensitivity in …
and quantification. Conventional DNA primers for PCR often suffer from poor sensitivity in …
Structural, optical and thermoelectric studies on chemically synthesized annealed antimony sulphide thin films
PA Chate, SD Lakde, DJ Sathe - Optik, 2022 - Elsevier
Antimony sulphide thin films have been developed by the dip method. The obtained thin
films were undergone heat treatment up to 473 K in the atmosphere. The variation in …
films were undergone heat treatment up to 473 K in the atmosphere. The variation in …
High-efficiency and high-fidelity ssDNA circularisation via the pairing of five 3′-terminal bases to assist LR-LAMP for the genotyping of single-nucleotide …
T Li, H Zou, J Zhang, H Ding, C Li, X Chen, Y Li… - Analyst, 2022 - pubs.rsc.org
The poor fidelity of T4 DNA ligase has always limited the simple detection of single-
nucleotide polymorphisms (SNPs) and is only applicable to some special SNP types. This …
nucleotide polymorphisms (SNPs) and is only applicable to some special SNP types. This …
[HTML][HTML] Allele-specific PCR for PIK3CA mutation detection using phosphoryl guanidine modified primers
AS Chubarov, IP Oscorbin, LM Novikova, ML Filipenko… - Diagnostics, 2023 - mdpi.com
Phosphoryl guanidine (PG) is the novel uncharged modification of internucleotide
phosphates of oligonucleotides. Incorporating PG modification into PCR primers leads to …
phosphates of oligonucleotides. Incorporating PG modification into PCR primers leads to …
Increasing the λ-Red mediated gene deletion efficiency in Escherichia coli using methyl phosphotriester-modified DNA
SC Chou, YJ Lai, XZ Zhuo, WY Chen, SY Li - Journal of the Taiwan Institute …, 2022 - Elsevier
Abstract Background Phage λ-Red recombineering is a powerful genetic tool to edit the
bacterial genome. With the demand of the multiplex genome editing, the efficiency of …
bacterial genome. With the demand of the multiplex genome editing, the efficiency of …
Specific and efficient knockdown of intracellular miRNA using partially neutralized phosphate-methylated DNA oligonucleic acid-loaded mesoporous silica …
YJ Sung, WT Cai, YP Chen, HWH Chan… - Journal of Materials …, 2024 - pubs.rsc.org
Antisense oligonucleotides (ASOs) are molecules used to regulate RNA expression by
targeting specific RNA sequences. One specific type of ASO, known as neutralized DNA …
targeting specific RNA sequences. One specific type of ASO, known as neutralized DNA …
OWL2: a molecular beacon-based nanostructure for highly selective detection of single-nucleotide variations in folded nucleic acids
BL Mueller, MJ Liberman, DM Kolpashchikov - Nanoscale, 2023 - pubs.rsc.org
Hybridization probes have been used in the detection of specific nucleic acids for the last 50
years. Despite the extensive efforts and the great significance, the challenges of the …
years. Despite the extensive efforts and the great significance, the challenges of the …