Cellular DNA replication is initiated through the action of multiprotein complexes that
recognize replication start sites in the chromosome (termed origins) and facilitate duplex …

The Mcm2-7 complex serves as the eukaryotic replicative helicase, the molecular motor that
both unwinds duplex DNA and powers fork progression during DNA replication. Consistent …

DNA replication in eukaryotes is strictly regulated by several mechanisms. A central step in
this replication is the assembly of the heterohexameric minichromosome maintenance …

Molecular motors are diverse enzymes that transduce chemical energy into mechanical
work and, in doing so, perform critical cellular functions such as DNA replication and …

Many complex cellular events depend on multiprotein complexes known as molecular
machines to efficiently couple the energy derived from adenosine triphosphate hydrolysis to …

Helicases are molecular machines that utilize energy derived from ATP hydrolysis to move
along nucleic acids and to separate base-paired nucleotides. The movement of the helicase …

DNA replication results in the doubling of the genome prior to cell division. This process
requires the assembly of 50 or more protein factors into a replication fork. Here, we review …

High-resolution structures have not been reported for replicative helicases at a replication
fork at atomic resolution, a prerequisite to understanding the unwinding mechanism. The …

DNA replication requires separation of genomic duplex DNA strands, an operation that is
performed by a hexameric ring-shaped helicase in all domains of life. The structures and …

During replication initiation, the core component of the helicase—the Mcm2-7 hexamer—is
loaded on origin DNA as a double hexamer (DH). The two ring-shaped hexamers are …