In the last decade, single-cell RNA sequencing routinely performed on large numbers of
single cells has greatly advanced our understanding of the underlying heterogeneity of …

MS-based proteome profiling has become increasingly comprehensive and quantitative, yet
a persistent shortcoming has been the relatively large samples required to achieve an in …

Quantitative proteomics employing mass spectrometry is an indispensable tool in life
science research. Targeted proteomics has emerged as a powerful approach for …

Data-independent acquisition approaches typically rely on experiment-specific spectrum
libraries, requiring offline fractionation and tens to hundreds of injections. We demonstrate a …

Great advances have been made in sensitivity and acquisition speed on the Orbitrap mass
analyzer, enabling increasingly deep proteome coverage. However, these advances have …

Mass spectrometry (MS) has progressed to become a powerful analytical tool for both
quantitative and qualitative applications. The first mass spectrometer was constructed in …

Single-cell proteomics (scProteomics) promises to advance our understanding of cell
functions within complex biological systems. However, a major challenge of current methods …

Shotgun proteomic data are affected by a variety of known and unknown systematic biases
as well as high proportions of missing values. Typically, normalization is performed in an …

Ion mobility can add a dimension to LC-MS based shotgun proteomics which has the
potential to boost proteome coverage, quantification accuracy and dynamic range. Required …

Nowadays, mass spectrometry‐based proteomics is carried out primarily in a bottom‐up
fashion, with peptides obtained after proteolytic digest of a whole proteome lysate as the …