Although they are often exploited to facilitate the expression and purification of recombinant
proteins, every affinity tag, whether large or small, has the potential to interfere with the …

Non-covalent interactions in the second coordination sphere of metalloenzymes play a
significant role in determining activity and selectivity. The importance of these interactions is …

Posttranslational modifications are an important feature of most proteases in higher
organisms, such as the conversion of inactive zymogens into active proteases. To date, little …

Carboxypeptidases (CPs) are a family of hydrolases that cleave one or more amino acids
from the C-terminal of peptides or proteins and play indispensable roles in various …

Fibrinolysis is initiated when the zymogen plasminogen is converted to plasmin via the
action of plasminogen activators. Proteolytic cleavage of fibrin by plasmin generates C …

Hydrogen bonding and other types of secondary-sphere interactions are ubiquitous in
metalloenzyme active sites and are critical to the transformations they mediate. Exploiting …

Carboxypeptidases (CPs) are a family of hydrolases that cleave one or more amino acids
from the C-terminal of peptides or proteins. However, methodology to monitor the activities of …

The use of fluorescent probes in a multitude of applications is still an expanding field. This
review covers the recent progress made in small molecular, spirocyclic xanthene-based …

Granzyme B (GrB) is a serine protease released by natural killer cells and cytotoxic T
lymphocytes during immune responses, which not only plays a role in tumor diagnosis but …

Metallo-carboxypeptidases are exopeptidases with diverse expression and function, found
in all kingdoms of life from bacteria to mammals. One of them, the carboxypeptidase A3 …