The diversity of conjugative relaxases and its application in plasmid classification
MP Garcillán-Barcia, MV Francia… - FEMS microbiology …, 2009 - academic.oup.com
Bacterial conjugation is an efficient and sophisticated mechanism of DNA transfer among
bacteria. While mobilizable plasmids only encode a minimal MOB machinery that allows …
bacteria. While mobilizable plasmids only encode a minimal MOB machinery that allows …
Conjugative DNA metabolism in Gram-negative bacteria
F De La Cruz, LS Frost, RJ Meyer… - FEMS microbiology …, 2010 - academic.oup.com
Bacterial conjugation in Gram-negative bacteria is triggered by a signal that connects the
relaxosome to the coupling protein (T4CP) and transferosome, a type IV secretion system …
relaxosome to the coupling protein (T4CP) and transferosome, a type IV secretion system …
Assembly and mechanisms of bacterial type IV secretion machines
EL Zechner, S Lang… - … Transactions of the …, 2012 - royalsocietypublishing.org
Type IV secretion occurs across a wide range of prokaryotic cell envelopes: Gram-negative,
Gram-positive, cell wall-less bacteria and some archaea. This diversity is reflected in the …
Gram-positive, cell wall-less bacteria and some archaea. This diversity is reflected in the …
Origin-of-transfer sequences facilitate mobilisation of non-conjugative antimicrobial-resistance plasmids in Staphylococcus aureus
FG O'Brien, K Yui Eto, RJT Murphy… - Nucleic acids …, 2015 - academic.oup.com
Staphylococcus aureus is a common cause of hospital, community and livestock-associated
infections and is increasingly resistant to multiple antimicrobials. A significant proportion of …
infections and is increasingly resistant to multiple antimicrobials. A significant proportion of …
Tracking F plasmid TraI relaxase processing reactions provides insight into F plasmid transfer
L Dostál, S Shao, JF Schildbach - Nucleic acids research, 2011 - academic.oup.com
Early in F plasmid conjugative transfer, the F relaxase, TraI, cleaves one plasmid strand at a
site within the origin of transfer called nic. The reaction covalently links TraI Tyr16 to the 5 …
site within the origin of transfer called nic. The reaction covalently links TraI Tyr16 to the 5 …
Structural basis of cooperative DNA recognition by the plasmid conjugation factor, TraM
The conjugative transfer of F-like plasmids such as F, R1, R100 and pED208, between
bacterial cells requires TraM, a plasmid-encoded DNA-binding protein. TraM tetramers …
bacterial cells requires TraM, a plasmid-encoded DNA-binding protein. TraM tetramers …
Conjugative DNA transfer is enhanced by plasmid R1 partitioning proteins
CJ Gruber, S Lang, VKH Rajendra, M Nuk… - Frontiers in Molecular …, 2016 - frontiersin.org
Bacterial conjugation is a form of type IV secretion used to transport protein and DNA directly
to recipient bacteria. The process is cell contact-dependent, yet the mechanisms enabling …
to recipient bacteria. The process is cell contact-dependent, yet the mechanisms enabling …
Relaxase DNA binding and cleavage are two distinguishable steps in conjugative DNA processing that involve different sequence elements of the nic site
M Lucas, B González-Pérez, M Cabezas… - Journal of Biological …, 2010 - ASBMB
TrwC, the relaxase of plasmid R388, catalyzes a series of concerted DNA cleavage and
strand transfer reactions on a specific site (nic) of its origin of transfer (oriT). nic contains the …
strand transfer reactions on a specific site (nic) of its origin of transfer (oriT). nic contains the …
Single-stranded DNA binding by F TraI relaxase and helicase domains is coordinately regulated
L Dostál, JF Schildbach - Journal of bacteriology, 2010 - Am Soc Microbiol
Transfer of conjugative plasmids requires relaxases, proteins that cleave one plasmid strand
sequence specifically. The F plasmid relaxase TraI (1,756 amino acids) is also a highly …
sequence specifically. The F plasmid relaxase TraI (1,756 amino acids) is also a highly …
A high security double lock and key mechanism in HUH relaxases controls oriT-processing for plasmid conjugation
JD Carballeira, B González-Pérez… - Nucleic acids …, 2014 - academic.oup.com
Relaxases act as DNA selection sieves in conjugative plasmid transfer. Most plasmid
relaxases belong to the HUH endonuclease family. TrwC, the relaxase of plasmid R388, is …
relaxases belong to the HUH endonuclease family. TrwC, the relaxase of plasmid R388, is …