Multigene amplification and massively parallel sequencing for cancer mutation discovery

F Dahl, J Stenberg, S Fredriksson… - Proceedings of the …, 2007 - National Acad Sciences
We have developed a procedure for massively parallel resequencing of multiple human
genes by combining a highly multiplexed and target-specific amplification process with a …

Nucleic acid amplification

LI Chieh-Yuan, D Ruff, SM Chen, J O'neil… - US Patent …, 2016 - Google Patents
2013-11-12 Assigned to Life Technologies Corporation reassignment Life Technologies
Corporation ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS) …

Nucleic acid amplification

LI Chieh-Yuan, D Ruff, SM Chen, J O'neil… - US Patent …, 2016 - Google Patents
2013-06-17 Assigned to Life Technologies Corporation reassignment Life Technologies
Corporation ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS) …

Hot Start PCR with heat-activatable primers: a novel approach for improved PCR performance

AV Lebedev, N Paul, J Yee, VA Timoshchuk… - Nucleic acids …, 2008 - academic.oup.com
The polymerase chain reaction (PCR) is widely used for applications which require a high
level of specificity and reliability, such as genetic testing, clinical diagnostics, blood …

Direct multiplex sequencing (DMPS)—a novel method for targeted high-throughput sequencing of ancient and highly degraded DNA

M Stiller, M Knapp, U Stenzel, M Hofreiter… - Genome …, 2009 - genome.cshlp.org
Although the emergence of high-throughput sequencing technologies has enabled whole-
genome sequencing from extinct organisms, little progress has been made in accelerating …

Novel approaches to mitigate primer interaction and eliminate inhibitors in multiplex PCR, demonstrated using an assay for detection of three strawberry viruses

T Wei, G Lu, G Clover - Journal of Virological Methods, 2008 - Elsevier
Multiplex PCR is an important technique for detecting a variety of pathogens simultaneously
in a single assay. Previous research has focused on optimising the factors affecting reliable …

Universal multiplex PCR: a novel method of simultaneous amplification of multiple DNA fragments

D Wen, C Zhang - Plant methods, 2012 - Springer
Background Multiplex PCR has been successfully applied in many areas since it was first
reported in 1988; however, it suffers from poor universality. Results A novel method called …

Multiplexed tandem PCR: gene profiling from small amounts of RNA using SYBR Green detection

KK Stanley, E Szewczuk - Nucleic acids research, 2005 - academic.oup.com
Abstract Multiplexed tandem PCR (MT-PCR) is a process for highly multiplexed gene
expression profiling. In the first step, multiple primer pairs are added to the RNA to be …

Microarray synthesis and assembly of gene-length polynucleotides

AV Oleinikov - US Patent 7,323,320, 2008 - Google Patents
There is disclosed a process for in vitro synthesis and assembly of long, gene-length
polynucleotides based upon assembly of multiple shorter oligonucleotides synthesized in …

Microarray synthesis and assembly of gene-length polynucleotides

AV Oleinikov - US Patent 7,563,600, 2009 - Google Patents
There is disclosed a process for in vitro synthesis and assembly of long, gene-length
polynucleotides based upon assembly of multiple shorter oligonucleotides synthesized in …