In eukaryotes, chromosome segregation during cell division is facilitated by the kinetochore,
a multiprotein structure that is assembled on centromeric DNA. The kinetochore attaches …

The selective degradation of many short-lived proteins in eukaryotic cells is carried out by
the ubiquitin system. In this pathway, proteins are targeted for degradation by covalent …

Between the 1960s and 1980s, most life scientists focused their attention on studies of
nucleic acids and the translation of the coded information. Protein degradation was a …

Cohesion between sister chromatids opposes the splitting force exerted by microtubules,
and loss of this cohesion is responsible for the subsequent separation of sister chromatids …

Cohesion between sister chromatids is established during DNA replication and depends on
a multiprotein complex called cohesin. Attachment of sister kinetochores to the mitotic …

▪ Abstract The separation of sister chromatids at the metaphase to anaphase transition is
one of the most dramatic of all cellular events and is a crucial aspect of all sexual and …

We describe a novel 25 kDa protein, geminin, which inhibits DNA replication and is
degraded during the mitotic phase of the cell cycle. Geminin has a destruction box sequence …

The S. cerevisiae MCD1 (mitotic chromosome determinant) gene was identified in genetic
screens for genes important for chromosome structure. MCD1 is essential for viability and …

In eukaryotic cells, replicated DNA strands remain physically connected until their
segregation to opposite poles of the cell during anaphase. This" sister chromatid cohesion" …

The proteolysis of key regulatory proteins is thought to control progress through mitosis.
Here we analyse cyclin B1 degradation in real time and find that it begins as soon as the last …