Highlights•Genome-scale models are available for many industrially important
fungi.•Enzyme-constrained genome-scale models improve simulation …

Constraint-based modeling (CBM) has evolved as the core systems biology tool to map the
interrelations between genotype, phenotype, and external environment. The recent …

Yarrowia lipolytica is an industrial yeast that can convert waste oil to value-added products.
However, it is unclear how this yeast metabolizes lipid feedstocks, specifically triacylglycerol …

Methanotrophy is the ability of an organism to capture and utilize the greenhouse gas,
methane, as a source of energy-rich carbon. Over the years, significant progress has been …

Finding the best knockout strategy for coupling biomass growth and production of a target
metabolite using a mathematic model of metabolism is a challenge in biotechnology. In this …

Motivation Genome-scale metabolic network reconstructions (GENREs) are valuable for
understanding cellular metabolism in silico. Several tools exist for automatic GENRE …

Fermentation-based biosynthesis in synthetic biology relies heavily on sugar-derived
feedstocks, a limited and carbon-intensive commodity. Unconventional feedstocks from less …

Constraint-based genome-scale models (GEMs) of microorganisms provide a powerful tool
for predicting and analyzing microbial phenotypes as well as for understanding how these …

Advances in synthetic biology and artificial intelligence (AI) have provided new opportunities
for modern biotechnology. High-performance cell factories, the backbone of industrial …

Over the past decades, virus‐like particle (VLP)‐based gene therapy (GT) evolved as a
promising approach to cure inherited diseases or cancer. Tremendous costs due to …