CRISPR-Cas9 viability screens are increasingly performed at a genome-wide scale across
large panels of cell lines to identify new therapeutic targets for precision cancer therapy …

Functional genomics describes a field of biology that uses a range of approaches for
assessing gene function with high-throughput molecular, genetic, and cellular technologies …

Simple Summary Cancer stem cells (CSCs) have been shown to be the main cause of
therapy resistance and cancer recurrence. An analysis of their biological properties has …

Genome-scale CRISPR-Cas9 viability screens performed in cancer cell lines provide a
systematic approach to identify cancer dependencies and new therapeutic targets. As …

Cell culture media are typically selected on the basis of common laboratory practices but
have major effects on the validity, reproducibility and physiological relevance of the scientific …

Targeting the distinct metabolic needs of tumor cells has recently emerged as a promising
strategy for cancer therapy. The heterogeneous, context-dependent nature of cancer cell …

CRISPR knockout fitness screens in cancer cell lines reveal many genes whose loss of
function causes cell death or loss of fitness or, more rarely, the opposite phenotype of faster …

Abstract Changes in cell metabolism and metabolic adaptation are hallmark features of
many cancers, including leukemia, that support biological processes involved into tumor …

Abstract Motivation Gene Set Enrichment Analysis (GSEA) is an algorithm widely used to
identify statistically enriched gene sets in transcriptomic data. However, GSEA cannot …

The metabolic reprogramming of cancer cells creates metabolic vulnerabilities that can be
therapeutically targeted. However, our understanding of metabolic dependencies and the …