iPSC-derived pancreatic progenitors lacking FOXA2 reveal alterations in miRNA expression targeting key pancreatic genes

N Aldous, AK Elsayed, NM Alajez… - Stem Cell Reviews and …, 2023 - Springer
Recently, we reported that forkhead box A2 (FOXA2) is required for the development of
human pancreatic α-and β-cells. However, whether miRNAs play a role in regulating …

p53 Inhibition in Pancreatic Progenitors Enhances the Differentiation of Human Pluripotent Stem Cells into Pancreatic β-Cells

II Aigha, EM Abdelalim - Stem cell reviews and reports, 2023 - Springer
The multipotent pancreatic progenitor cells (MPCs) co-expressing the transcription factors,
PDX1 and NKX6. 1, are the source of functional pancreatic β-cells. The aim of this study was …

Microfluidic chip systems for characterizing glucose-responsive insulin-secreting cells equipped with FailSafe kill-switch

M Izadifar, M Massumi, KJ Prentice, T Oussenko… - Stem Cell Research & …, 2024 - Springer
Background Pluripotent cell-derived islet replacement therapy offers promise for treating
Type 1 diabetes (T1D), but concerns about uncontrolled cell proliferation and tumorigenicity …

Identifying miRNA Signatures Associated with Pancreatic Islet Dysfunction in a FOXA2-Deficient iPSC Model

AK Elsayed, N Aldous, NM Alajez… - Stem Cell Reviews and …, 2024 - Springer
The pathogenesis of diabetes involves complex changes in the expression profiles of mRNA
and non-coding RNAs within pancreatic islet cells. Recent progress in induced pluripotent …

Differentiation of human pluripotent stem cells into pancreatic beta-cell precursors in a 2D culture system

B Memon, EM Abdelalim - JoVE (Journal of Visualized Experiments), 2021 - jove.com
Human pluripotent stem cells (hPSCs) are an excellent tool for studying early pancreatic
development and investigating the genetic contributors to diabetes. hPSC-derived insulin …

Reprogramming of DPSC to Induced Pluripotent Stem Cells

S Yildirim - Dental Pulp Derived Mesenchymal Stromal Cells, 2024 - Springer
A primary objective in cell biology research is to induce a true regenerative response from
pluripotent cells, which have the potential to develop into virtually any cell type. Takahashi …