Surveying membrane landscapes: a new look at the bacterial cell surface

T Lithgow, CJ Stubenrauch, MPH Stumpf - Nature Reviews Microbiology, 2023 - nature.com
Recent studies applying advanced imaging techniques are changing the way we
understand bacterial cell surfaces, bringing new knowledge on everything from single-cell …

Outer membrane porins contribute to antimicrobial resistance in gram-negative bacteria

G Zhou, Q Wang, Y Wang, X Wen, H Peng, R Peng… - Microorganisms, 2023 - mdpi.com
Gram-negative bacteria depend on their cell membranes for survival and environmental
adaptation. They contain two membranes, one of which is the outer membrane (OM), which …

A periplasmic phospholipase that maintains outer membrane lipid asymmetry in Pseudomonas aeruginosa

RL Guest, MJ Lee, W Wang… - Proceedings of the …, 2023 - National Acad Sciences
The outer membrane of Gram-negative bacteria is unique in both structure and function. The
surface-exposed outer leaflet is composed of lipopolysaccharide, while the inner leaflet is …

Darobactin B Stabilises a Lateral‐Closed Conformation of the BAM Complex in E. coli Cells

SF Haysom, J Machin, JM Whitehouse… - Angewandte Chemie …, 2023 - Wiley Online Library
The β‐barrel assembly machinery (BAM complex) is essential for outer membrane protein
(OMP) folding in Gram‐negative bacteria, and represents a promising antimicrobial target …

Tunable force transduction through the Escherichia coli cell envelope

DP Williams-Jones, MN Webby… - Proceedings of the …, 2023 - National Acad Sciences
The outer membrane (OM) of Gram-negative bacteria is not energised and so processes
requiring a driving force must connect to energy-transduction systems in the inner …

Mechanism of outer membrane destabilization by global reduction of protein content

IV Mikheyeva, J Sun, KC Huang, TJ Silhavy - Nature communications, 2023 - nature.com
The outer membrane (OM) of Gram-negative bacteria such as Escherichia coli is an
asymmetric bilayer with the glycolipid lipopolysaccharide (LPS) in the outer leaflet and …

Molecular Machines that Facilitate Bacterial Outer Membrane Protein Biogenesis

MT Doyle, HD Bernstein - Annual Review of Biochemistry, 2024 - annualreviews.org
Almost all outer membrane proteins (OMPs) in Gram-negative bacteria contain a β-barrel
domain that spans the outer membrane (OM). To reach the OM, OMPs must be translocated …

A dual detergent strategy to capture a bacterial outer membrane proteome in peptidiscs for characterization by mass spectrometry and binding assays

JW Young, Z Zhao, IS Wason… - Journal of Proteome …, 2022 - ACS Publications
The outer membrane of Gram-negative bacteria plays a critical role in protecting the cell
against external stressors, including antibiotics, and therefore is a prime target for …

[HTML][HTML] Computational microbiology of bacteria: Advancements in molecular dynamics simulations

S Khalid, AF Brandner, N Juraschko, KE Newman… - Structure, 2023 - cell.com
Microbiology is traditionally considered within the context of wet laboratory methodologies.
Computational techniques have a great potential to contribute to microbiology. Here, we …

Reply to: Antibiotics and hexagonal order in the bacterial outer membrane

S Manioglu, SM Modaresi, J Thoma, SA Overall… - Nature …, 2023 - nature.com
Methods AFM imaging and analysis OMVs produced from different E. coli MG1655 strains
were adsorbed onto freshly cleaved mica for 15min in DPBS buffer at room temperature …