Protein phosphorylation is a post-translational modification (PTM) that orchestrates a diverse
array of cellular processes. Because this modification serves as a rapid and reversible …

After 35 years of development, immobilized metal ion affinity chromatography (IMAC) has
evolved into a popular protein purification technique. This review starts with a discussion of …

Phosphorylation is a form of protein posttranslational modification (PTM) that regulates many
biological processes. Whereas phosphoproteomics is a scientific discipline that identifies …

The highly specific enrichment of phosphoproteins and phosphopeptides from intricate
biological systems is the precondition of in-depth phosphoproteome research. Herein, a …

Phosphopeptide enrichment is an essential step in large-scale, quantitative
phosphoproteomics by mass spectrometry. Several phosphopeptide affinity enrichment …

Advances in phosphopeptide enrichment methods enable the identification of thousands of
phosphopeptides from complex samples. Current offline enrichment approaches using TiO …

Phosphoproteomics has become one of the most active research areas in proteomics
studies. Phosphopeptide enrichment is a critical and indispensable step in …

Methodologies to enrich heterogeneous types of phosphopeptides are critical for
comprehensive mapping of the under-explored phosphoproteome. Taking advantage of the …

Large-scale protein phosphorylation analysis by MS is emerging as a powerful tool in plant
signal transduction research. However, our current understanding of the phosphorylation …

Metal ions differed greatly in affinity towards phosphopeptides, and thus it is essential to
systematically compare the phosphopeptides enrichment ability of different metal ions …